月见草油微乳冻干粉性能研究

Performance Study on Microemulsion Lyophilized Powder of Evening Primrose Oil

研究冻干保护剂对月见草油微乳冻干粉性能的影响并对冻干粉稳定性进行考察。采用DSC法分析微乳冻干粉中苏丹Ⅱ的存在形式;以外观性状、粒径、再分散性为指标对微乳冷冻干燥中所使用的冻干保护剂进行筛选;以包封率为指标考察贮藏温度和时间对冻干粉稳定性的影响。结果显示以甘露醇为冻干保护剂的月见草油微乳冻干粉形态好复水速度快,复溶后粒径变大。冻干粉在37℃下贮存6个月后,微乳包封率从原来的90.92±0.79%下降为82.58±0.66%,变化显著;4℃和20℃下贮存包封率分别为89.50±0.76%、88.80±0.98%,下降较少。DSC数据显示苏丹Ⅱ的加入对微乳冻干粉的热性能没有影响,苏丹Ⅱ以无定形形式存在于冻干粉中。以月见草油为油相,以甘露醇为冻干保护剂制备的微乳冻干粉性质稳定,包封率较高,在室温下贮存6个月无明显变化。

To investigate the effects of cryoprotectants on the freeze-drying of evening primrose oil microemulsion and its stability,the distribution of Sudan II in the lyophilized powder was analyzed by differential scanning calorimeter（DSC）.The cryoprotectants for the microemulsion were screened in terms of appearance,size and powder disperse ability.The stability of the powder under various conditions of storage time and temperature was investigated with the entrapment efficiency as the observing index.In summary,mannitol was shown to be the best cryoprotectant.The lyophilized powder showed good granular pattern,dispersion speed,and large-sized particles.After storage for six months,the entrapment efficiency was decreased from 90.92% to 82.58±0.66% at 37 ℃;while it only decreased to 89.50% and 88.80% at 4 ℃and 20 ℃,respectively.DSC results showed that Sudan II had no effect on thermal property of the microemulsion lyophilized powder and existed as amorphous pattern.The microemulsion lyophilized powder,prepared using evening primrose oil as the oily phase and mannitol as the lyoprotectant,was stable,with high entrapment efficiency and no significant change after stored for 6 months.