山奈酚对糖尿病大鼠骨髓来源内皮祖细胞管腔形成的影响

Effects of kaempferol on tube formation of endothelial progenitor cells in diabetic rats

目的观察山奈酚对糖尿病大鼠骨髓来源内皮祖细胞管腔形成能力的影响,为2型糖尿病的治疗提供参考依据。方法体外原代培养内皮祖细胞,采用牛血清白蛋白糖基化终末产物(BSA-AGEs)(100mg/L)与山奈酚(2μM,10μM,50μM)分别作用于内皮祖细胞24h,M^atrigel胶上观察其管腔形成能力。结果山奈酚(2μM,10μM,50μM)三组的EPCs管腔形成数目分别是4.7/×100视野,5.2/×100视野和6.1/×100视野,与AGEs组(4.3/×100视野)比较,组间比较差异有统计学意义(F=55.58,P<0.05)。结论山奈酚能够改善糖尿病内皮功能障碍,提高微血管管腔形成的能力,对2型糖尿病性心肌病的治疗和糖尿病血管并发症的预防有效。

Objective To observe the effects of kaempferol on tube formation in endothelial progenitor cells in rats and provide reference for treament of type 2 diabetes. Methods The endothelial progenitor cells in rats were cultured in vitro with kaempferol 2μM, 10μM and 50μM respectively, AGEs （100mg/l） was added and resumed incubation for 24 hours. The tube formation in endothelial progenitor cells of rats in every groups were determined. Results The number of tube formation of endothelial progenitor cells observed under 100 microscopy field in （2μM,10μM,50μM kaempferol group were 4.7/100 fields,5.2//100fields and 6.1/100fields,showing significant difference between groups（F=55.58,P〈0.05）. Conclusion Kaempferol may be capable of suppressing activation of oxidative stress pathway in endothelial progenitor cells and promoting tube formation .Thus it is effective for treatment of diabetic myocardopathy and diabetes associated vascular complications.