摘要
目的分析转化生长因子-β(TGF-β)和血管内皮生长因子-C(VEGF-C)在原发性肝细胞癌(PHC)中的表达及其临床意义。方法应用SP免疫组织化学法检测TGF-β和VEGF-C在PHC组患者及正常组肝组织中的表达情况,并用酶联免疫吸附试验法检测两组患者血清TGF-β和VEGF-C含量,并进行对比分析。结果 56例PHC组肝组织的VEGF-C和TGF-β阳性表达率分别是76.8%(43/56)、92.8%(52/56),60例正常组的阳性表达率分别是3.36%(2/60)、10.0%(6/60),二者之间的VEGF-C和TGF-β阳性表达率均有统计学差异(P<0.05)。PHC组血清TGF-β和VEGF-C含量分别为(290.8±65.8)pg/mL,(372.6±86.3)pg/mL,正常组血清TGF-β和VEGF-C含量分别为(70.1±25.6)pg/mL,(81.3±16.5)pg/mL,差异均有统计学意义(P<0.05)。结论 TGF-β和VEGF-C异常表达与PHC的发展密切相关,可以提高PHC的诊断率,对预测PHC肿瘤侵袭转移及判断预后具有一定价值。
Objective To analyze the clinical significance of detection of TGF-β and VEGF-C in patients with primary hepatocellular carcinoma(PHC). Methods Expression of TGF-β and VEGF-C in PHC group (56 cases of PHC patients) and normal group (60 cases of normal people) in liver tissue were detected by SP-immune histochemical method. Serum content of TGF-β and VEGF-C were measured by ELISA and compared between two groups. Results Positive expression rate of VEGF - C and TGF - beta in PHC group were 76.8% (43/56) and 92.8% (52/56), while those in normal group were 3.36% (2/60) and 10.0% (6/60), showing statistical difference between the two groups(P〈0.05). Serum content of TGF-β and VEGF-C content in PHC group were (290.8±65.8) pg/ml and (372.6±86.3)pg/mL; while those in normal group were (70.1±25.6) pg / ml and (81.3±16.5) pg / ml,revealing statistical significant difference between the two groups (P〈0.05). Conclusion Abnormal expression of TGF-β and VEGF-C is closely related to the development of PHC, thus the detection of TGF-β and VEGF-C can improve the diagnostic rate of PHC, which plays an role in prediction of PHC tumor invasion and metastasis and the prognosis judgment.
出处
《中国热带医学》
CAS
2014年第8期1000-1001,共2页
China Tropical Medicine