渴络欣胶囊的血清药物化学研究

Study on Serum Pharmacochemistry of Ke-Luo-Xin Capsule

目的:研究渴络欣胶囊吸收入血的主要物质基础及特征。方法:采用HPLC-DAD体系,建立渴络欣胶囊大鼠血清的HPLC指纹图谱;差示分析渴络欣胶囊、空白血清及含药血清指纹图谱,获得大鼠口服渴络欣胶囊后血液中移行成分概况。结果:所建大鼠血清指纹图谱方法具有良好的精密度、重复性和稳定性;基于该方法分析渴络欣胶囊大鼠含药血清,共检出移行成分13个,其中原型成分9个、代谢成分4个,原型成分中包括芦荟大黄素及大黄酸。结论:吸收入血的13种成分可能是渴络欣胶囊发挥药效的主要物质基础。

This article was aimed to study the main effective substances and characteristics of Ke-Luo-Xin （KLX） capsule. HPLC-DAD system was applied in the establishment of HPLC finger prints of serum of rats after taking KLX capsule. And then, serum samples taken KLX capsule, blank blood serum, and single crude drugs were com-pared. Constituents absorbed into the serum were determined by HPLC-DAD system. The results showed that meth-ods for serum HPLC fingerprinting had good precision, reproducibility and stability. A total of 13 constituents migrat-ing to the blood were detected, of which 9 were prototype constituents, 4 were metabolites. Prototype constituents in-cluded aloeemodin and chrysophanic acid. It was concluded that 13 constituents migrating to the blood may be the main effective substances of KLX capsule.