黄芪对维生素D缺乏大鼠骨质的保护作用

Protective Effect of Astragalus on Bones of Vitamin D Deficiency Rats

目的:观察黄芪对维生素D缺乏大鼠骨质的保护作用。方法:选用30只雄性SD大鼠,随机分为3组:对照组、模型组、黄芪组。实验周期8周。实验结束后,酶免法检测血清25羟维生素D3[25(OH)VD3]、成纤维生长因子-23[FGF-23]、Klotho,股骨HE染色。结果:与正常对照组比较,维生素D缺乏大鼠模型组血清25(OH)VD3、Klotho降低,FGF-23升高,存在骨质疏松。与模型组比较,黄芪组血清25(OH)VD3、FGF-23降低,Klotho升高;黄芪组骨质疏松明显改善。结论:黄芪具有调节维生素D缺乏大鼠FGF-23、Klotho,保护骨质作用。

This study was aimed to observe the protective effect of astragalus on bones of vitamin D deficiency rat model. A total of 30 male SD rats were randomly divided into 3 groups, which were the control group, model group, and the astragalus group. The experimental period was 8 weeks. After the experiment, enzyme immunoassay was used for the detection of serum 25 hydroxy vitamin D3 [25 （OH）VD3], fibroblast growth factor-23 [FGF-23], Klotho, and HE staining of femur. The results showed that compared with the normal control group, the vitamin D deficiency rat model group had a decrease in both serum 25 （OH）VD3 and Klotho, and a increase in FGF-23, which meant the ex-istence of osteoporosis. Compared with the model group, the astragalus group had a decrease in both the serum 25 （OH）VD3 and FGF-23, and a increase in Klotho, which meant the osteoporosis of the astragalus group had been im-proved significantly. It was concluded that astragalus can regulate FGF-23 and Klotho in vitamin D deficiency rats in order to have a protective effect for bones.