补阳还五汤精简方对脑缺血模型大鼠神经功能及脑梗死面积的影响

Effects of the Thin Recipe of Buyanghuanwu on Neurological Function and Area of Cerebral Infarction in Cerebral Ischemic Rat

目的探讨补阳还五汤精简方对缺血性脑损伤的保护作用。方法采用大脑中动脉线栓法建立大脑中动脉闭塞模型,实验大鼠随机分为假手术组、模型组、补阳还五汤组和补阳还五汤精简方高、中、低剂量组,各给药组给予相应药物灌胃,模型组和假手术组给予等容积无菌蒸馏水,连续7 d。运用Matlab软件编程分析数据,观察各组大鼠神经功能、体质量、脑梗死面积和脑干湿重的变化。结果脑缺血后大鼠出现大面积脑梗死、脑组织水肿和神经功能障碍。与模型组比较,补阳还五汤精简方中、低剂量组均能改善模型大鼠的各项指标,差异有统计学意义(P<0.05)。结论补阳还五汤精简方对脑缺血损伤具有保护作用,给药量以中剂量最佳。

Objective To study the protective effect of the thin Recipe of Buyanghuanwu on cerebral ischemic injury of rats. Methods Block model in cerebral artery was established through using the suture method of middle cerebral artery. The rats were randomly divided into sham-operation group, model group, Buyanghuanwu group and thin recipe of Buyanghuanyin high, medium and low-dose groups. Buyanghuanwu group and thin recipe of Buyanghuanyin groups were given gavage with relevant medicine, and sham-operation group and model group were given gavage with the same amount of sterile distilled water for continuous 7 days. Matlab software programming was used to analyze data and observe the changes in neurological function, body weight, the area of cerebral infarction and wet/dry weight ratio of brain of the rats. Results Rats with cerebral ischemia suffered large area of infarction, brain edema and neurological dysfunction. The thin recipe of Buyanghuanwu of medium and low dose groups could effectively improve the indexes of model rats compared with the model group, the difference with statistical significance （P〈0.05）. Conclusion The thin recipe of Buyanghuanwu has protective effect on cerebral ischemia, and the medium dose is the optimal dose.