抵当芪桂汤对糖尿病大鼠胰岛炎症及β细胞结构的影响

Effects of Didang Qigui Recipe on Islet Inflammation and Structure of Pancreatic β Cell in Streptozotocin-induced Diabetic Rats

目的探讨抵当芪桂汤防治糖尿病的作用机制。方法 80只雌性SD大鼠随机抽取10只设为空白组,其余70只大鼠腹腔内一次性注射链脲佐菌素60 mg/kg造模,选取成模大鼠50只随机分为模型组、中药常量组、西药组、中药大剂量组、中药常量+格列齐特组(中西药组),每组10只,各给药组予相应药物灌胃。8周后,测定血清中白细胞介素(IL)-1β、IL-2的含量,并观察胰岛β细胞结构的变化。结果与模型组比较,各给药组大鼠血清IL-1β、IL-2含量均降低,其中以中药常量组、中西药组较为明显(P<0.05);中药常量组、中西药组胰岛β细胞凋亡程度较轻(P<0.05)。结论抵当芪桂汤通过抑制胰岛炎症反应,保护胰岛β细胞结构而达到防治糖尿病的作用。

Objective To explore the mechanism of Didang Qigui Recipe on preventing diabetes. Methods Ten female rats were set randomly as normal group, and others 70 were injected with STZ （60 mg/kg） to establish the diabetic model. After the model was established, 50 model rats were randomly divided into five groups：the model group, Didang Qigui Recipe group, the gliclazide group, Didang Qigui Recipe high-dose group, Didang Qigui Recipe and gliclazide group, with 10 rats in each group. All groups were given a gavage with related medicine. With treatment being given respectively, eight weeks later, contents of IL-1βand IL–2 in serum were detected by ELISA and structure of pancreatic β cells was observed by immunohistochemistry and microscope. Results Compared with the model group, the contents of IL-1βand IL-2 in serum of rats decreased in treatment groups, especially in Didang Qigui Recipe group and Didang Qigui and gliclazide group （P〈0.05）. The degree of apoptosis in pancreaticβcells, especially in the above two groups, has been less affected than other groups （P〈0.05）. Conclusion Didang Qigui Recipe has preventive and therapeutic effects on diabetes by inhibition of inflammatory response and protection structure of pancreaticβcells.