离子色谱法测定大蒜多糖的单糖组成和含量

Determination of Composition and Content of Monoses in Garlic Polysaccharide by Ion Chromatography

目的建立测定大蒜多糖的单糖组成和含量的离子色谱法。方法以氨基酸分离柱,氢氧化钠/醋酸钠溶液梯度洗脱,脉冲积分安培检测器（Au为工作电极,Ag/AgCl为参比电极）检测,测定大蒜多糖水解产生的单糖组成及含量。结果大蒜多糖主要由阿拉伯糖、半乳糖、葡萄糖、木糖、果糖等单糖组成,各单糖基本达到分离,线性关系良好,相关系数均〉0.998,平均回收率均在95%～105%之间。结论所建立的方法准确、简单,可用于大蒜多糖中单糖的含量测定和质量控制。

Objective To establish a method to analyze the composition and content of monoses in the garlic polysaccharide by ion chromatography. Methods The monoses in the solution of garlic polysaccharide hydrolysised by trifluoroacetic acid were determined by amino PAC column, eluted by gradient hydroxide sodium and identified by ampere detector （AU as working electrode, Ag/AgCl as reference electrode）. Results The garlic polysaccharide was mainly composed by arabinose, galactose, glucose, xylose and fructose. Good resolution could be achieved among the above mentioned five kinds of monoses. Their linear correlations over the investigated concentration were above 0.998, while the average recovery rates were in the range of 95%-105%. Conclusion The ion chromatography method is fast, accurate, simple and reliable, and can be applied in the content determination and quality control of garlic polysaccharide.