胃腺癌中survivin和EGFR的作用及其相互关系

Role of the survivin and EGFR in gastric cancer and their correlation

目的探讨survivin及表皮生长因子受体EGFR在胃癌中的表达作用及二者的相关性。方法应用免疫组织化学的方法检测60例胃腺癌中survivin及EGFR的表达,采用MTT法观察EGFR抑制剂AG1478对胃癌细胞生长的影响,FCM法检测细胞凋亡,蛋白质印迹分析方法检测survivin的表达变化。结果 EGFR和survivin在胃癌组织中的表达显著高于在正常胃组织中的表达(P<0.05),并且EGFR与survivin的表达具有相关性(r=0.587,P<0.005);EGFR抑制剂AG1478能够抑制胃癌细胞SGC7901的增殖,并能够诱导凋亡,并下调survivin的表达。结论 survivin和EGFR在胃癌中的表达具有相关性,并且survivin可能在EGFR引起的肿瘤细胞增殖中发挥着重要作用。survivin和EGFR有望成为胃癌治疗的联合靶点。

Objective To explore the role of EGFR and survivin in the gastric cancer and their correlation.Methods The expression of EGFR and survivin was measured in gastric adenocarcinoma by immnohistochemistry.Human gastric cancer cell line SGC-7901 was cultured and treated with different concentrations of AG1478,then the proliferation of SGC-7901 cells was determined by MTT,the apoptosis was detected by FCM,and the survivin expression was determined by Western blot.Results The expression of EGFR and survivin protein was significantly higher in gastric adenocarcinoma tissues than those in normal gastric epithelium.And there was positive correlation between EGFR and survivin expression in gastric adenocarcinoma.The AG1478 inhibited the cell proliferation,increased the apoptosis of SGC7901,and downregulated the expression of survivin.Conclusion The results suggest that survivin may play an important role in tumor cell proliferation induced by EGFR.Survivin and EGFR are promising to be a combined therapeutic strategy in gastric cancer.