十溴联苯醚(BDE-209)对中华哲水蚤的急性毒性效应及体内三种抗氧化酶活性的影响

Acute toxic effects of decabromodiphenyl ether( BDE-209) and its effects on three kinds of antioxidant enzymes in copepod Calanus sinicus

多溴联苯醚(PBDEs)是一类具有潜在生态风险的新型持久性有机污染物,在环境和生物体中含量均呈现增长趋势。本文研究了十溴联苯醚(BDE-209)对中华哲水蚤96 h-LC50以及不同浓度BDE-209胁迫下中华哲水蚤体内三种抗氧化酶:谷胱甘肽过氧化物酶(GPX)、谷胱甘肽巯基转移酶(GST)和谷胱甘肽还原酶(GR)响应变化。实验结果显示:(1)BDE-209对中华哲水蚤96 h-LC50值为1 432.21μg/L,表明BDE-209是一种高毒性物质。(2)BDE-209可对中华哲水蚤造成一定程度的氧化胁迫。低浓度组(20μg/L)GPX、GST、GR活性随胁迫时间增加而升高,体现了机体抗氧化防御系统对BDE-209胁迫的应激反应;高浓度组(200μg/L)GST、GR活性在96 h高于对照组,而GPX活性始终低于对照组,表明机体受到一定氧化损伤。

Polybrominated diphenyl ethers （PBDEs）, a group of new type of persistent organic pollutants （POPs） with potential ecological risk, are now widely distributed in the environment and organisms. In this study, acute toxic test of BDE-209 on copepod Calanus sinicus was carried out to measure the 96 h-LC50. Results showed that the 96 h-LC50 of BDE-209 to C. sinicus was 1 432.21 μg/L which belonged to highly toxic material. The response of three kinds of antioxidant enzymes： glutathione peroxidase （GPx ）, glutathione-S-transferase （GST） and glutathione reductase （GR） when exposed to different concentrations of BDE-209 for 48 and 96 h were also investigated in the present study. BDE-209 could induce oxidative stress of C. sinicus and the enzymic activity of GPx, GST and GR in low-concentration （20 μg/L） group rose with time increasing, reflecting responses of defense system to the stress. In the highconcentration group （200 μg/L） the activity of GST and GR increased at the time of 96 h, while the activity of GPx was always lower than those of the control, showing the occnrance of certain oxidative damage.