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两组冷冻保护剂对卵裂期胚胎玻璃化冷冻效果的研究

Effect of vitrification on cleavage stage embryos using two groups of cryoprotectants
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摘要 目的评价两组玻璃化冷冻保护剂对卵裂期胚胎的冷冻效果。方法以体外受精-胚胎移植周期患者受精后第3天废弃胚胎为实验对象,共102枚胚胎入选,随机分为乙二醇+丙二醇(EG+PROH)组52枚,乙二醇+二甲基亚砜(EG+DMSO)组50枚。比较观察两组玻璃化冷冻保护剂对卵裂期胚胎冷冻解冻后胚胎的复苏效果。结果玻璃化冻融后两组在复苏率上无显著差异,但在完整胚胎复苏率和卵裂球复苏率上EG+PROH组显著高于EG+DMSO组(P<0.05)。结论对于卵裂期胚胎的玻璃化冷冻,选用EG+PROH作为冷冻保护剂可取得较好的效果。 Objective To evaluate the effects of two groups of cryoprotectants on cleavage stage embryos . Methods A total of 102 discarded embryos on day 3 after fertilizaion from IVF/ICSI-ET cycles were randomly allo-cated to group of ethylene glycol ＋propanediol（EG＋PROH） （52 embryos）and group of ethylene glycol ＋dimethyl sulphoxide（EG＋DMSO）（50 embryos）.After warming, the survivals efficient of embryos between the two groups were compared.Results After warming, the two groups had a similar survival rate , but the intact survival rate and blastomere survival rate of cleavage stage embryos in the EG ＋PROH group was significantly higher than those in the EG＋DMSO group（P〈0.05）.Conclusion For the vitrification of cleavage stage embryos , using EG＋PROH as a cryoprotectant can achieve good results .
作者 王世凯 黄莉 何冰 谭卫红 薛林涛 成俊萍 覃捷
机构地区 广西壮族自治区人民医院生殖医学与遗传中心
出处 《中国临床新医学》 2014年第9期806-809,共4页 CHINESE JOURNAL OF NEW CLINICAL MEDICINE
基金 广西卫生厅科研课题(编号:Z2013343)
关键词 玻璃化冷冻 冷冻保护剂 卵裂期胚胎 Vitrification Cryoprotectant Cleavage stage embryo
分类号 R711.6 [医药卫生—妇产科学]
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参考文献17

  • 1Mukaida T, Wads S,Takahashi K, et al. Vitrification of human embry- os based on the assessment of suitable conditions for 8-cell mouse em- bryos[ J]. Hum Reprod, 1998,13 ( 10 ) :2874 - 2879.
  • 2薛林涛,黄莉,何冰,谭卫红,王世凯,成俊萍,覃捷,刘睿智.玻璃化冷冻人活检后不同发育时期胚胎的研究[J].中国妇幼保健,2013,28(36):6010-6013. 被引量：6
  • 3Balaball B, Urman B, Ata B, et al. A randomized controlled study of human Day 3 embryo cryopreservation by slow freezing or vitrification : vitrification is associated with higher survival, metabolism and blasto- cyst formation [ J ]. Hum Rcprod ,2008,23 ( 9 ) : 1976 - 1982.
  • 4Chian R C,Kuwayama M,Tan L,et al. High survival rate of bovine o- ocytes matured in vitro following vitrification [ J ]. J Reprod Dev, 2004,50 (6) :685 - 696.
  • 5Yoon TK, Chung HM, Lim JM,et al. Pregnancy and delivery of healthy infants developect from vitrified oocytes in a stimulates irtvitro fertiliza- tion embryo transfer program [ J ]. Fertil Steril, 2000,74 ( 1 ) : 180 - 181.
  • 6Newton H , Fisher J , Arnold J R P , et al. Permeation of hnman ovari- an tissue with eryoproteetive agent s in preparation for eryopreservation [ J ]. Hum Reprod, 1998,13 (2) :376 - 380.
  • 7Kartberg A J, Hambiliki F,Arvidsson T,et al. Vitrification with DMSO protects embryo membrane integrity Detter than solutions wilhout DM-SO [ J ]. Reprod Biomed Online,2008,17 ( 3 ) :378 - 384.
  • 8Sheehan CB, Lane M, Gardner DK. The CryoLoop facilitates re-vitrifi- cation of embryos at four successive stages of development without im- pairing embryo growth [ J ]. Hum Reprod, 2006,21 ( 11 ) : 2978 - 2984.
  • 9Desai N,Blackmon H, Szeptycki J, et al. Cryoloop vitrification of hu- man day 3 cleavage-stage embryos: post-vitrification development, pregnancy outcomes and live births [ J ]. Reprod Biomed Online, 2007,14(2) :208 -213.
  • 10Kasai M,Mukaida T. Cryopreservation of animal and human embryos by vitrification [ J ]. Reprod Biomed Online,2004,9 ( 2) : 164 - 170.

二级参考文献46

  • 1Burns W,Gandet T,Martin M,et al.Survival of cryopreservation and thawing with all blastomeres intact identifies multicell embryos with superior frozen embryo transfer outcome[J].Fertil Steril,1999,72:527-532.
  • 2Vanderzwalmen P,Berlin G,Debauehe CH,et al.Births after vitrification at monda and blastoeyst stages:effect of artificial reduction of the blastocoelic cavity before vitrification[J].Hum Reprod,2002,17(3):744-751.
  • 3Mukaida T,Wada S,Takahashi K,et al.Vitrification of human embryos based on the assessment of suitable conditions for 8-cell mouse embryos[J].Hum Reprod,1998,13:2874-2879.
  • 4Massip A.Cryopreservation of embryos of farm animals[J].Reprod Domest Anita,2001,36:49-55.
  • 5Kasai M.Vitrification:refined strategy for the cryopreservation of mammalian embryos[J].Mamm Ova Res,1997,14:17-28.
  • 6Kasai M,Mukaida T.Cryopreservation of animal and human embryos by vitrification[J].Reprod Biomed Online,2004,9:164-170.
  • 7Scott LA;Smith S.The successful use of pronuclear embryo transfers the day following oocyte retrieval[J],1998(04).
  • 8Gardner DK;Lane M;Stevens J.Blastocyst score affects implantation and pregnancy outcome:towards a single blastocyst transfer[J],2000(06).
  • 9Twisk M;Mastenbroek S;van Wely M.Preimplantation genetic screening for abnormal number of chromosomes (aneuploidies) in in vitro fertilization or intracytoplasmic sperm injection,2006(01).
  • 10Alikani M;Calderon G;Tomkin G.Cleavage anomalies in early human embryos and survival after prolonged culture in-vitro[J],2000(12).

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中国临床新医学
2014年 第9期

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