五味子甲素对铅染毒大鼠睾丸谷胱甘肽等生化指标的影响

Effects of Schisandrin A on Some Biochemical Indicators Such As Glutathione in Rat Testes with Low Lead Exposed

目的:探讨五味子甲素对低铅染毒大鼠睾丸谷胱甘肽等生化指标的影响,以探寻其对抗氧化作用的影响。方法:将18只健康SPF级SD雄性大鼠随机分作3组:空白对照组、醋酸铅组及醋酸铅与五味子甲素组,每组6只。灌胃处理,10周末,检测每组大鼠的血铅含量、睾丸谷胱甘肽转移酶(GST)、还原型谷胱甘肽(GSH)及氧化型谷胱甘肽(GSSG)的含量。结果:与空白对照组比较,两处理组中血铅含量均明显升高(P<0.05);两处理组中GST含量与GSH/GSSG均明显减少(P<0.05)。与醋酸铅组比较,醋酸铅与五味子甲素组中GST含量与GSH/GSSG均有增加,且GSH/GSSG差异有统计学意义(P<0.05)。结论:五味子甲素能对低铅染毒大鼠睾丸的抗氧化起到一定作用。

Objective： To detect the effects of Schisandrin A on some biochemical indicators such as glutathione in rat testes with low lead exposed, in order to explore the role of its antioxidant effects. Method： The 18 healthy SPF male SD rats were randomly and averagely divided into 3 groups, control group, lead acetate group and lead acetate with Schisandrin A group,6 in each. After gavage treatment for 10 weeks, Pb2＋ content of blood, testicular glutathione S-transferase（GST）, reduced glutathione（GSH） and oxidized glutathione（GSSG） levels were all measured in each group of rats. Results： Compared with control group, Pb2＋ content of blood were both significantly higher（P〈0.05） and GST content and GSH/GSSG were both significant decrease（P〈0.05） in the two treatment groups. And compared with lead acetate group, GST content and GSH/GSSG were both increased, and the difference of GSH/GSSG was statistically significant（P〈0.05） in lead acetate with Schisandrin A group. Conclusion： Schisandrin A could provide a certain effects on antioxidant in the rat testes of low lead exposure.