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南宁壮族人群17个STR基因座遗传多态性研究 被引量:2

Genetic Polymorphism of 17 STR Loci in Nanning Zhuang Population
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摘要 目的探讨17个短串联重复序列(STR)基因座在南宁壮族人群中的等位基因频率分布及群体遗传学参数。方法应用PowerPlex 18D System和ABI3130遗传分析仪,对596名南宁壮族、无关个体的血DNA进行多态性研究。结果在南宁壮族人群中,17个基因座的基因型分布经χ^2检验符合Hardy-Weinberg平衡,个体识别概率0.781 0-0.977 7,三联非父排除率0.297 8-0.783 8,基因座偶合率0.022 3-0.219 0,多态性信息总量为0.540 1-0.879 1,17个基因座累积个体识别能力〉0.999999999999。结论 PowerPlex 18D System在南宁壮族人群的个体识别和亲权鉴定中具有较高的应用价值。 Objective To explore the allele frequency and population genetics parameters of the 17 short tandem repeat(STR) loci in Nanning Zhuang population. Methods PowerPlex 18 D System and ABI3130 genetic analyzer were used to analyze the DNA polymorphism of the blood samples collected from 596 individuals of Nanning Zhuang population. Results The genotype distribution of the 17 STR loci,which was analyzed by χ^2test,accorded with Hardy-Weinberg equilibrium in Nanning Zhuang population. The power of discrimination ranged from 0. 781 0 to0. 977 7,the triple probability of paternity exclusion ranged from 0. 297 8 to 0. 783 8,the loci matching probability ranged from 0. 022 3 to 0. 219 0,the polymorphism information content ranged from 0. 540 1 to 0. 879 1,the total probability of discrimination power of the 17 STR loci was greater than 0. 999 999 999 999. Conclusion PowerPlex 18 D System has a higher application value in the individual discrimination identification and paternity test of Nanning Zhuang population.
出处 《广西医学》 CAS 2014年第10期1360-1362,共3页 Guangxi Medical Journal
基金 广西自然科学基金资助项目(2010GXNSFA013262)
关键词 亲权鉴定 个体识别 短串联重复序列 多态性 南宁市 壮族 Short tandem repeat Individual identification Paternity test Polymorphism Nanning City Zhuang ethnic group
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二级参考文献10

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