剑麻皂素对大鼠局灶性脑缺血再灌注损伤的保护作用及其机制

Protective Effect of Tigogenine on Rats with Focal Cerebral Ischemia-Reperfusion Injury and Its Mechanism

目的探讨剑麻皂素对大鼠局灶性脑缺血再灌注损伤的保护作用及其机制。方法采用大脑中动脉闭塞法制备局灶性脑缺血再灌注损伤大鼠模型,模型建立成功后,选取大鼠60只,按随机数字表法分为模型组、三七通舒组,剑麻皂素高、中、低剂量组,每组12只。模型组灌服蒸馏水,三七通舒组灌服三七通舒胶囊,剑麻皂素高、中、低剂量组分别按450、225、113 mg/(kg·d)灌服剑麻皂素。对比各组神经行为学评分、脑梗死体积、脑组织含水量及血清超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、乳酸(LA)水平。结果剑麻皂素高、中剂量组大鼠神经行为学评分明显低于模型组(P<0.05),绝对脑梗死体积和相对脑梗死体积明显小于模型组(P<0.05),剑麻皂素高剂量组脑含水量明显低于模型组(P<0.05)。剑麻皂素高剂量组大鼠血清SOD、GSH-Px水平明显高于模型组,MDA、LA水平明显低于模型组(P<0.05);剑麻皂素中剂量组大鼠血清SOD水平明显高于模型组(P<0.05),MDA水平低于模型组(P<0.05)。结论剑麻皂素对大鼠局灶性脑缺血再灌注损伤具有明显保护作用,其机制可能与清除自由基、减轻LA性酸中毒程度有关。

Objective To explore the protective effects of tigogenine on rats with focal cerebral ischemia-reperfusion injury and its mechanism. Methods Copied the model of focal cerebral ischemia reperfusion in rats by middle cerebral artery occlusion（MCAO）. Then 60 rats were randomly divided into model group,Sanqitongshu group,high-,middle- and low-dose tigogenine groups,with 12 rats in each group. The model group was given distilled water by gavage,Sanqitongshu group was given Sanqitongshu capsule by gavage,high-,middle- and low-dose tigogenine groups were given tigogenine of450 mg /（kg·d）,225 mg /（kg·d）and 113 mg /（kg·d）,respectively,by gavage. The neuroethology score,volume of cerebral infarction,cerebral water content and the serum superoxide dismutase（SOD）,malonaldehyde（MDA）,glutathione peroxidase（GSH-Px）,lactic acid（LA） levels were compared among groups.Results The neuroethology score of high-and middle-dose tigogenine groups was significantly lower than that of model group（P〈0.05）,the absolute and relative volumes of ischemia of high- and middle-dose tigogenine groups were significantly smaller than those of model group（P〈0. 05）,the cerebral water content of high-dose tigogenine group was significantly lower than that of model group（P〈0.05）. The serum SOD and GSH-Px levels of high-dose tigogenine group were significantly higher than those of model group while the MDA and LA levels were significantly lower than those of model group（P〈0. 05）. The serum SOD level of middle-dose tigogenine group was significantly higher than that of model group（ P〈0. 05）,but the MDA level was lower than that of model group（P〈0.05）. Conclusion Tigogenine has the protective effect on rats with focal cerebral ischemia-reperfusion injury,which might be related with anti-free radical,anti-oxidation function and relieving lactic acidosis.