2型糖尿病患者外周血中膜型及可溶性CD28分子的表达及意义

The significance and expression of serum membrane type CD28 and soluble CD28 of among 2 diabetes patients

目的分析2型糖尿病患者外周血中膜型及可溶性CD28分子的表达及其意义。方法分别收集2012年12月至2013年12月在北京军区联勤部门诊部就诊的30例2型糖尿病患者(糖尿病组)及同期30名健康体检者(对照组)的外周血,通过溶血及流式细胞术检测其外周血T细胞表面CD28分子的表达,酶联免疫吸附(ELISA)法检测血清中可溶性CD28的水平,并进行对比分析。结果糖尿病组外周血CD4+CD28+T细胞和CD8+CD28+T细胞显著低于对照组[(28.29±7.71)%比(32.77±4.28)%和(12.18±3.17)%比(18.53±3.27)%,均P<0.01],血清中可溶性CD28水平显著高于对照组[(2.86±0.96)比(0.61±0.19)μg/L,P<0.01]。结论协同刺激分子CD28很可能参与了2型糖尿病的免疫病理机制。

Objective To analyze the expression of membrane type CD28 and soluble CD28 in peripheral blood of type 2 diabetes patients. Methods The peripheral blood mononuclear cells（ PBMCs） of 30 subjects with type 2 diabetes（ diabetes group） and 30 healthy donors（ control group） were collected from December 2012 to December 2013 in the out- patient department of Outreach Logistics Department of Beijing Military Region. The expression of CD28 on T cells in peripheral blood of type 2 diabetes patients was detected by flow cytometry（ FCM）. The secretion of soluble CD28 in serum was detected using the skills of Enzyme- linked immunosorbent assay（ ELISA）.The data was analyzed by the Graphpad Prism 5. 01 program. Results The percentage of CD4^ ＋ CD28^ ＋ and CD8^＋ CD28 ^＋ T cells in peripheral blood of type 2 diabetes patients were（ 28. 29 ± 7. 71） % and（ 12. 18 ± 3. 17） % respectively,and were decreased markedly compared with healthy control（ 32. 77 ± 4. 28% and 18. 53 ± 3. 27%,P 0. 01 and P 〈0. 001）. Compared with control,the level of soluble CD28 was increased significantly in serum of type2 diabetes patients（（ 2. 86 ± 0. 96） vs（ 0. 61 ± 0. 19） μg /L,P 〈0. 001）. Conclusion The costimulatory molecule CD28 maybe take part in the immunological pathogenesis of type 2 diabetes.