1,3-二氨基胍盐酸盐的高效液相色谱分析

Determination of 1,3-diaminoguanidine monohydrochloride by HPLC

提出用高效液相色谱法测定1，3-二氨基胍盐酸盐的含量，选用甲醇与乙酸铵缓冲盐体积比75：25为流动相，c8色谱柱，检测波长314nm。试验结果表明：测试结果的相对标准偏差小于2．03％；回收率在97．8％～100．2％；1，3-二氨基胍盐酸盐和三氨基胍盐酸盐得到较好的分离。该法操作简单、实用且定量分析结果准确稳定。

The content of 1,3-diaminoguanidine monohydroehloride is determined by HPLC using chromatographic column packed with C8. The mobile phase is methanol and ammonium acetate buffer salt with volume ratio of 75 ： 25. The detection wave length is at 254 nm. The results show that the standard deviation is less than 2. 03%, and the recovery is between 98.0%～ 100. 1%. 1,3-Diamin- oguanidine monohydrochloride is effectively separated from triaminoguanidine monohydroehloride. This method has advantages of simple operation, practical and accuracy.