成人隐匿性自身免疫性糖尿病与溃疡性结肠炎患者外周血T细胞亚群失衡的临床意义

The peripheral blood T cell subsets imbalance in patients with latent autoimmune diabetes in adult and ulcerative colitis and its clinical significance

目的探讨LADA与溃疡性结肠炎(UC)患者外周血T细胞亚群变化及淋巴细胞失衡的临床意义。方法选取LADA、UC组各52例和健康对照(NC)组52名,采用单克隆抗体间接免疫荧光技术检测各组外周血淋巴细胞CD3+、CD4+、CD8+亚群百分比。结果 LADA、UC、NC组CD3+亚群比较差异均无统计学意义[(62.29±4.97)%vs(63.49±5.12)%vs(61.37±4.83)%,P>0.05]。UC组CD4+亚群较LADA、NC组降低[(27.63±6.85)%vs(35.87±5.92)%,P<0.01;(27.63±6.85)%vs(34.63±5.39)%,P<0.01]。LADA组CD8+亚群较UC、NC组升高[(27.54±3.05)%vs(22.21±3.46)%,P<0.01;(27.54±3.05)%vs(21.82±3.77)%,P<0.01]。LADA、UC组CD4+/CD8+较NC组降低[(1.35±0.37)vs(1.78±0.44),P<0.01;(1.32±0.51)vs(1.78±0.44),P<0.01]。结论 LADA、UC患者均出现淋巴细胞CD4+、CD8+亚群失衡,淋巴细胞亚群失衡参与两者发病,但具体机制不同。

Objective To detect changes of peripheral blood T cell subsets in patients with LADA and ulcerative colitis （UC） in order to investigate the clinical significance of T cell subsets imbalance. Methods 52 cases of LADA, 52 cases of UC and 52 normal control （NC） people were selected, and peripheral blood T lymphocyte CD3^＋, CD4^＋, CD8^＋ subsets were quantified by an indirect immtmofluorescence technique using monoclonal antibodies. Results There were no statistically difference in the percentage of CD3^＋ subsets among three groups [（62.29±4.97）% vs （63.49±5.12）% vs （61.37±4.83）%, P〉0. 05]. The percentage of CD4^＋ subsets was lower in UC group than in LADA and NC group [（27.63±6.85）% vs （35.87±5.92）%,P〈0.01; （27.63±6.85）% vs （34.63±5.39）%,P〈0.01]. The percentage of CD8^＋ subsets was higher in LADA group than in UC and NC group [（27.54±3.05）% vs （22.21±3.46）%,P〈0. 01; （27.54±3.05） % vs （21.82±3.77）% ,P〈0.01]. The ratio of CD^4＋/CD8^＋ was higher in LADA group and UC group than in NC group [（1.35±0.37） vs （1.78±0.44）,P〈0.01; （1.32±0.51） vs （1.78±0.44）,P〈0. 01]. Conclusion The results CD4＋/CD8＋ lymphocytes imbalance in LADA and UC suggest that lymphocyte subsets imbalance is involved in the incidence of both LADA and UC,but the extensional pathogenesis is incomplete the same.