HPLC测定芩暴红止咳胶囊中东莨菪内酯、黄芩苷、黄芩素和杜鹃素的含量

Determination of Scopoletin, Baicalin, Baicalein and Farrerol in Qinbaohong Zhike Capsule by HPLC

目的采用HPLC测定芩暴红止咳胶囊中东莨菪内酯、黄芩苷、黄芩素、杜鹃素的含量。方法 Eclipse XDB-C18色谱柱（4.6 mm×250 mm,5μm）,流动相：甲醇（A）-1%冰醋酸溶液（B）,梯度洗脱,流速：0.8 mL·min-1;检测波长：295 nm;柱温：30℃。结果东莨菪内酯线性范围为1.27~124.70μg,r=0.999 9,加样回收率为98.3%（RSD=1.1%）;黄芩苷线性范围为8.07~538.20μg,r=1.000 0,样品加样回收率为98.0%（RSD=1.2%）;黄芩素线性范围为1.12~111.60μg,r=1.000 0,加样回收率为98.5%（RSD=0.9%）;杜鹃素线性范围为1.06~106.40μg,r=1.000 0,加样回收率为99.0%（RSD=1.4%）。结论本方法简便、准确,重复性好,专属性高,可用于制剂的质量控制。

OBJECTIVE To establish a method for determining the content of scopoletin, baicalin, baicalein and farrerol in Qinbaohong Zhike capsule. METHODS An Eclipse XDB-C18 column （4.6 mm×250 mm,5μm） was used with methanol （A）-1% acetic acid （B） as the mobile phase at the flow rate of 0.8 mL-min-1, and the UV-detection wavelength was set at 295 nm with the column temperature of 30 ℃. RESULTS Scopoletin showed a good linear relationship at the range of 1.27-124.70 μg （r=0.999 9）, the average recovery was 98.3% with RSD of 1.1%. Baicalin showed a good linear relationship at the range of 8.07-538.20 μg（r=1.000 0）, the average recovery was 98.0% with RSD of 1.2%. Baicalein showed a good linear relationship at the range of 1.12-111.60 μg（r=1.000 0）, the average recovery was 98.5% with RSD of 0.9%. Farrerol showed a good linear relationship at the range of 1.06-106.40 μg（r=l.000 0）, the average recovery was 99.0% with RSD of 1.4%. CONCLUSION This method is simple, accurate and reproducible. It can be reliable for the quality control of Qinbaohong Zhike Capsule.