维生素E与镁对肥胖大鼠糖脂代谢影响的研究

Effects of vitamin E and magnesium on glucolipid metabolism in obese rats

目的观察维生素E(VE)与镁(Mg)对肥胖大鼠糖脂代谢的影响。方法健康Wistar大鼠共74只随机分为5组:正常对照组、高脂高糖组、高脂高糖+VE组、高脂高糖+Mg组和高脂高糖+VE+Mg组。VE和Mg的剂量分别为0.23 g/kg和0.31 g/kg饲料。实验喂养67周处死动物,计算脂肪体重比;检测血脂水平;检测血糖、胰岛素水平,计算胰岛素敏感指数;分析己糖激酶、丙酮酸激酶活性。各结果采用单因素方差分析及析因设计方差分析进行统计。结果高脂高糖组大鼠的体重比正常对照组明显高20%,肥胖大鼠模型建立成功。干预67周后,高脂高糖+VE+Mg组脂体比为13.29%、血浆甘油三酯为0.6 mmol/L,明显低于高脂高糖组的17.24%和1.18 mmol/L(P<0.05),且高脂高糖+VE+Mg组甘油三酯水平明显低于高脂高糖+VE组的1.07 mmol/L(P<0.05);高脂高糖+VE、高脂高糖+VE+Mg组的己糖激酶活性分别为63.67 U/L和64.61 U/L,较高脂高糖组42.79 U/L和高脂高糖+Mg组44.02 U/L明显增高(P<0.05)。结论联合补充VE与Mg可以有效地改善肥胖大鼠脂肪含量及甘油三酯代谢,且降低甘油三酯的作用较单纯补充VE效果显著。此外,其还可以提高肥胖大鼠己糖激酶的活性,且添加VE组较单纯补充Mg组效果更佳。

Objective To investigate the effects of vitamin E （VE） and magnesium （Mg） supplementantion on glucolipid metabolism in obese rats. Methods Seventy-four healthy Wistar rats were randomly divided into five groups （normal control group positive control group VE group, Mg group, VE plus Mg group）. The doses of VE and Mg were 0.23 g/kg feed and 0. 31 g/kg feed respectively. They were slaughtered after feed for 67 weeks. Calculate the lipid ratio. Examined the levels of serum lipid, plasma glucose, serum insulin, insulin sensitivity index, and the activity of hexokinase and pyruvate kinase. The results of each group were analyzed by One-Way ANOVA and analysis of variance of factorial design. Results The rat model with nutritional obesity wassuccessfully established that the weight of positive control group was about 20% higher than normal control group. The lipid ratio and plasma triglyceride of VE plus Mg group were 13.29% and 0.6 mmol/L respectively, which were significantly lower than that of positive control group （ 17.24% and 1.18 mmol/L） （P 〈 0.05）, and the plasma triglyceride of VE plus Mg group was significantly lower than that of VE group （ 1.07mmol/ L） （P 〈 0.05）. The activity of hexokinase of VE group and VE plus Mg group were 63.67 U/L and 64.61 U/L respectively, significantly higher than that of positive control group （42.79 U/L） and Mg group （44.02 U/L） （P 〈 0.05）. Conclusion Supplementation of VE combined with Mg can effectively improve the fat content and triglyceride of obese rats, better than VE alone. Supplementation of VE and Mg is beneficial to obese rats to improve the activity of hexokinase, and adding VE is better than Mg alone.