正交试验优选复方牛黄胶囊的提取工艺

Optimization of Extraction Process of Compound Niuhuang Capsules

目的:优选复方牛黄胶囊的提取工艺。方法:采用UV测定总皂苷含量,检测波长分别为560,545 nm。以总皂苷质量为评价指标,选择加水量、浸泡时间、煎煮时间、煎煮次数为考察因素,采用L9(34)正交试验优选复方牛黄胶囊的水提工艺;选择乙醇体积分数、乙醇用量、提取时间及提取次数为考察因素,采用L9(34)正交试验优选醇提工艺。结果:最佳水提条件为加10倍量水浸泡0.5 h,煎煮3次,每次2 h;最佳醇提工艺为加8倍量70%乙醇煎煮3次,每次2 h。结论:优化的提取工艺稳定可行,为复方牛黄胶囊的临床推广提供实验依据。

Objective： To optimize extraction process of compound Niuhuang capsules. Method： UV was employed to determine the content of total saponins with detection wavelength at 560 nm and 545 nm. Taking quality of total saponins as index, orthogonal test was adopted to optimize water extraction process with the amount of water, soaking time, extration time and times as factors; the same method was used to investigate effects of ethanol concentration, ethanol amount, extraction time and times on alcohol extraction process. Result： The best water extraction process was： soaked 0.5 h with 10 times the amount of water, decocted two hours for three times; optimum alcohol extraction process was as following： decocted thrice with 8 times the amount of 70% ethanol for 2 h each time. Conclusion： These extraction process is stable and feasible, this study can provide an experimental basis for clinical application of compound Niuhuang capsules.