HPLC测定栀子不同炮制品中栀子苷、绿原酸和西红花苷-Ⅰ含量

Determination on Contents of Geniposide,Chlorogenic Acid and Crocin-Ⅰfrom Different Processed Products of Gardeniae Fructus by HPLC

目的：比较栀子不同炮制品水提取部位中栀子苷、绿原酸和西红花苷-Ⅰ含量的差异,探索其炮制原理.方法：按2010年版《中国药典》标准炮制工艺制备生栀子、炒栀子和焦栀子,加水回流提取制备供试品溶液,采用HPLC-DAD检测栀子苷、绿原酸和西红花苷-Ⅰ含量,检测波长分别为240,330,440 nm,流动相甲醇-乙腈（9∶1,A）-0.3％甲酸水溶液（B）梯度洗脱（0～15 min,10％ ～30％A;15～20 min,30％A;20～60 min,30％～65％A;60～65 min,65％A;65～75 min,65％ ～10％A）,流速0.8 mL· min-1.结果：生栀子、炒栀子和焦栀子中栀子苷、绿原酸和西红花苷-Ⅰ质量分数均依次降低,栀子苷分别为2.84％,2.33％,1.57％,绿原酸依次为0.13％,0.09％,0.04％,西红花苷-Ⅰ分别为0.18％,0.16％,0.11％.结论：栀子不同炮制品水提部分主要药效成分—栀子苷、绿原酸和西红花苷-Ⅰ的含量存在差异,推测这与其炮制后缓和苦寒之性、增强凉血止血功效相关.

Objective： To compare content differences of geniposide, chlorogenic acid and crocin-Ⅰ from water extracts of different processed products of Gardeniae Fructus for exploring its processing principle. Method： Crude, stir-fired and stir-baked products of Gardeniae Fructus was prepared by standard processing technology in the 2010 edition of ＇ Chinese Pharmacopoeia＇ , then extracted by heat reflux water-extraction, HPLC-DAD was employed to determine contents of geniposide, chlorogenic acid and crocin- Ⅰ with detection wavelength of 240, 330, 440 nm, respectively; mobile phase of methanol-acetonitrile （9 ： 1, A） and 0.3% formic acid aqueous solution （B） for gradient elution （0-15 min, 10%-30% A; 15-20 min, 30% A; 20-60 rain, 30%-65%A; 60-65 min, 65% A; 65-75 min, 65% -10% A） and flow rate of 0.8 mL ·min^-1. Result： Contents of three ingredients from the crude, stir-fired and stir-baked products were reduced in turn; geniposide content were 2.84% , 2.33% , 1.57% ; chlorogenic acid content were 0.13% , 0.09% , 0.04% ; crocin- Ⅰ content were 0. 18% , 0. 16% , 0. 11% , respectively. Conclusion： Contents of main medicinal ingredients in water extracts of different processed products exist differences, speculating that it may be processing principle of easing properties of bitter-cold, enhancing cold-blood and hemostatic effect after processing.