摘要
目的:采用高效液相-电喷雾四级杆飞行时间串联质谱(HPLC-ESI-Q-TOF-MS)技术对淫羊藿水提取部分中的化学成分进行定性分析。方法:采用液相色谱-串联质谱(LC-MS-MS),C18色谱柱(4.6 mm×250 mm,5μm)以甲醇-0.1%甲酸溶液为流动相梯度洗脱;质谱使用ESI离子源,正离子与负离子模式下采集数据。结果:通过正、负离子质谱信息及元素组成,分析与相关文献数据对照,共鉴定出10个化合物。结论:通过超高效液相色谱分离,质谱测定相对分子质量及相关文献数据信息检索确定淫羊藿水提取部分中的化学成分,为阐明淫羊藿水提部分的药效物质基础提供有力的证据。
Objective： This study aimed to conduct a qualitative analysis on chemical components in water extract of Epimedii Folium by high performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry （HPLC-ESI-Q-TOF-MS）. Method： LC-MS-MS was adopted, using C18 （4.6 mm × 250 mm, 5 μm） column with methanol-0. 1% formic acid as the mobile phase for gradient elution; ESI ion source was used for mass spectra, and data were collected in positive and negative mode. Result： Ten compounds from water extract of Epimedii Folium were identified by analyzing positive and negative ion mass spectra information and element composition, and comparing controls of data from relevant literatures. Conclusion ： After the separation by ultra high performance liquid chromatography, relative molecular mass was determined by mass spectra, and chemical compounds in water extract of Epimedii Folinm were determined by information search through relevant literature data, in order to provide powerful therapeutic material basis for water extract of Epimedii Folium.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第19期112-116,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江苏高校优势学科建设工程资助项目(2011ZYX6-014)
江苏省2012年度普通高校研究生科研创新计划项目(CXZZ12-0626)
江苏省中医药局科技项目(LZ13013)
