生、熟延胡索饮片药理作用的对比研究

Comparative Study Between Crude Corydalis Rhizoma and Vinegar Corydalis Rhizoma in Pharmacological Action

目的:基于张仲景"生熟异用"理论探讨延胡索及其炮制品的药效差异,阐明中药炮制理论的科学性,为临床合理使用延胡索提供参考。方法:采用热板法与醋酸扭体实验研究生、醋延胡索的镇痛作用;采用二甲苯致耳廓肿胀模型研究生、醋延胡索对昆明种小鼠耳廓肿胀度的影响;采用离体肠平滑肌运动实验研究生、醋延胡索对Wistar大鼠离体肠平滑肌运动的影响;采用比浊法研究生、醋延胡索对ADP、胶原诱导的Wistar大鼠血小板聚集的影响。结果:生、醋延胡索对热传导、化学刺激引起的拟痛反应较对照组存在显著差异且生、醋延胡索之间存在显著性差异(P<0.05,P<0.01);生、醋延胡索较对照组均能显著抑制二甲苯致耳廓肿胀度(P<0.01),但生、醋延胡索之间无差异;醋延胡索较生延胡索可明显抑制Wistar大鼠离体肠平滑肌舒缩活动(P<0.05,P<0.01);生、醋延胡索在抗ADP、胶原诱导的血小板聚集方面无差异。结论:延胡索醋制可增强镇痛和解痉作用,符合中药炮制生熟论的"生效熟增"的原则。在临床上以汤剂用药时,针对痛症,应用醋延胡索饮片入药,以增强镇痛和解痉作用。

Objective： To clarify the rationality of traditional Chinese medicine after processing and to provide references for using rational them in the clinic, the pharmacodynamic differences of raw Corydalis Rhizoma and vinegar Corydalis Rhizoma based on the theory of crude and cooked traditional Chinese medicine decoction pieces using in different ways advocated by ZHANG Zhong-jing was investigated respectively. Method： By mice hot-board and acetic-acid-induced twisting experiments, the analgesic effect of raw Corydalis Rhizoma and vinegar Corydalis Rhizoma was observed. By mice auricular swelling model by injecting xylene in the abdomen of mice, the anti-inflammation effect of raw Corydalis Rhizoma and vinegar Corydalis Rhizoma on kunming mouse was observed ; by the contractile activity on isolated rat small intestine smooth muscle, the spasmolysis effect of raw Corydalis Rhizoma and vinegar Corydalis Rhizoma on Wistar rat was observed; by turbidimetry method, the anti-platelet aggregation effect induced by ADP or collagen on Wistar rat was observed. Result： Compared to blank group, raw Corydalis Rhizoma and vinegar Corydalis Rhizoma for the analgesic effects caused by the heat conduction and chemical stimulation had obvious difference, and there were also obvious difference between raw Corydalis Rhizoma and vinegar Corydalis Rhizoma （P 〈 0.05, P 〈 0.01 ）. Compared to blank group, raw Corydalis Rhizoma and vinegar Corydalis Rhizoma could inhibit the swelling degrees induced by injecting xylene （P 〈 0.01 ） , but there were no difference between raw Corydalis Rhizoma and vinegar Corydalis Rhizoma; compared to raw Corydalis Rhizoma, vinegar Corydalis Rhizoma could inhibit the contractile activity of isolated rat small intestine smooth muscle （P 〈 0.05, P 〈 0.01 ）. There were no difference between raw Corydalis Rhizoma and vinegar Corydalis Rhizoma on anti-platelet aggregation induced by ADP and collagen. Conclusion： It indicates that Corydalis Rhizoma after processing with vinegar can improve analgesic and spasmolysis effects, corresponding the Chinese medicine processing regulation that the Chinese medicine after processing can increase effects of raw drugs. Using vinegar Corydalis Rhizoma in prescriptions can strengthen analgesic and spasmolysis effects for pain symptoms in the clinic.