基质金属蛋白酶及其抑制因子在宫颈癌中的表达及临床意义

Expressions and significance of matrix metalloproteinase and tissu inhibitor of matrix metallopro-teinase in cervical cancer

目的：探讨基质金属蛋白酶2（MMP-2）和MMP-9及其抑制因子1（TIMP-1）和TIMP-2在宫颈癌中的表达及其临床意义。方法选取经病理证实的早期宫颈浸润鳞癌患者98例（研究组）、宫颈上皮内瘤样病变（ CIN）患者68例（ CIN组），取病变中心组织和边缘组织；同时选取正常宫颈组织标本36例为对照组。应用逆转录-聚合酶链反应（ RT-PCR）检测各组中MMP-2、MMP-9、TIMP-1和TIMP-2表达阳性率和表达水平，比较各组相关因子表达情况的差异性。结果对照组中 MMP-2和 MMP-9的阳性率和表达水平显著低于 CIN 组和研究组， TIMP-1和TIMP-2的阳性率和表达水平显著高于CIN组和研究组（ P﹤0.05）。CIN组中MMP-2和MMP-9的阳性率和表达水平显著低于研究组，TIMP-1和TIMP-2的阳性率和表达水平显著高于研究组（ P﹤0.05）。研究组的边缘癌组织中MMP-2与MMP-9的阳性率和表达水平明显高于中心癌组织，而TIMP-1与TIMP-2的阳性率和表达水平明显低于中心癌组织（ P﹤0.05）。结论 MMP-2、MMP-9及其抑制因子TIMP-1、TIMP-2与宫颈癌的发生、发展密切相关，可能在宫颈癌的侵袭与转移中发挥着重要作用。

Objective To investigate the expressions and significance of matrix metalloproteinase （ MMP-2 ,MMP-9 ）and tissue inhibitor of matrix metalloproteinase（ TIMP-2 ,TIMP-1 ）in cervical cancer. Methods Ninety-eight patients with early invasive cervical cancer（ ICC）and 68 patients with cervical intraepithelial neoplasm（ CIN）,confirmed by pathology,were selected,and 36 cases of normal cervical epithelium（NCE）were selected as control. The expression of MMP-2,MMP-9,TIMP-2 and TIMP-1 in all samples were examined by reverse transcription PCR. Expression rate and expression level of ev-ery factor were recorded. The results were compared among the groups. Results Compared with control group,the expression rate and expression levels of MMP-2,MMP-9 in ICC group and CIN group were significantly higher,TIMP-2 and TIMP-1 in ICC group and CIN group were significantly lower（ P ﹤0. 05）. Expression rate and expression levels of MMP-2,MMP-9 in CIN group were significantly lower than those in ICC group,TIMP-2 ,TIMP-1 in CIN group were significantly higher than those in ICC group（ P﹤0. 05 ）. The expression levels of MMP-2 ,MMP-9 in the tumor edge of the ICC group were significantly higher than those in the tumor center,while the expression levels of TIMP-2 ,TIMP-1 were significantly lower than those in the tumor center（ P ﹤0. 05 ）. Conclusions MMP-2 ,MMP-9 and TIMP-2 ,TIMP-1 may play an important role in the development,progression,invasion and metastasis of cervical cancer.