摘要
目的:建立维生素C泡腾片中有关物质含量的测定方法。方法:采用高效液相色谱法,色谱柱为PerfectChrom100C18(250mm×4.6mm,5μm);流动相为磷酸盐缓冲溶液(PH2.50±0.05);流速:0.8ml/min;进样量:20μl;检测波长为245 nm。结果:维生素C质量浓度在4.0~16.0μg/ml范围内,进样量在0.0618~0.5562μg范围内与峰面积线性关系良好(r=0.9990)。结论:该方法简便准确,稳定性好,可用于维生素C泡腾片中有关物质的含量测定。
Objective: to develop a quantitative method for the determination of related substances in the VitC Effervescent.Methods: PerfectChrom 100 C18(250 mm× 4.6 mm,5 μm) was used as chromatographic column,phosphate buffer(PH2.50 ± 0.05) was used as mobile phase; the injection vohtme was 20μl and the detection wavelength was at 245nm with a flow rate of 0.8 ml/min.Results: The standard working curve of VC and peak area had a good linear relation between the concentration from 4.0 to 16.0 μg·mL-1 and the injection volume from 0.0618 to 0.5562 μg (r = 0.9990); Conclusion: This method is simple, precision and stable which is can be used for the determination of related substances in the VitC Effervescent.
出处
《山东食品发酵》
2014年第3期34-37,共4页
Shandong Food Ferment