Y盒结合蛋白1与上皮-间质转化标志物在结直肠癌中表达的研究

Expression of Y-box-binding protein-1 and epithelial-mesenchymal transition markers in colorectal cancer

目的：检测结直肠癌组织中 Y 盒结合蛋白1与上皮-间质转化标志物（E 钙黏蛋白和 N 钙黏蛋白）的表达，分析 Y 盒结合蛋白1与临床病理参数之间的关系并评价其与 E 钙黏蛋白、N 钙黏蛋白之间的相关性。方法用蛋白免疫印迹和免疫组织化学染色法检测120例结直肠癌组织和相对应的癌旁正常组织中 Y 盒结合蛋白1、E 钙黏蛋白和 N 钙黏蛋白表达情况，并对其结果进行分析。结果结直肠癌组织中 Y 盒结合蛋白1、E 钙黏蛋白和 N 钙黏蛋白表达情况与癌旁正常组织相比差异有统计学意义（χ2=47.373，P ﹤0.05；χ2=83.145，P ﹤0.05；χ2=41.832，P ﹤0.05）。结直肠癌组织中 Y 盒结合蛋白1表达情况与肿瘤分化程度、肿瘤侵犯、淋巴结转移及远处转移有关（χ2=8.077，P =0.008；χ2=8.178， P =0.006；χ2=15.152，P ﹤0.001；χ2=7.368，P =0.011），与 E 钙黏蛋白表达呈负相关（r =-0.238，P =0.009），与 N 钙黏蛋白表达呈正相关（r =0.361，P ﹤0.001）。结论 Y 盒结合蛋白1可能通过参与上皮-间质转化促进结直肠癌的发生发展。

Objective To detect the expressions of Y-box-binding protein-1（YB-1）and epithelial-mesenchymal transition（EMT）markers（E-cadherin and N-cadherin）in colorectal cancer（CRC）,to analyze the relationship between the expression of YB-1 and clinicopathological parameters,to evaluate the correlations among YB-1,E-cadherin and N-cadherin. Methods The expressions of YB-1,E-cadherin and N-cadherin in 120 primary CRC tumors and corresponding normal tissues were detected by western blot and immunohistochem-istry and the results were analyzed. Results The expressions of YB-1,E-cadherin and N-cadherin in tumors were significantly different from those in corresponding normal tissues（χ2 = 47. 373,P ﹤ 0. 05;χ2 = 83. 145, P ﹤ 0. 05;χ2 = 41. 832,P ﹤ 0. 05）. The expression of YB-1 in tumors was associated significantly with tumor differentiation,tumor invasion,lymph node metastasis and distance metastasis（χ2 = 8. 077,P = 0. 008;χ2 =8. 178,P = 0. 006;χ2 = 15. 152,P ﹤ 0. 001;χ2 = 7. 368,P = 0. 011）. It was negatively correlated with E-cadherin expression（r = - 0. 238,P = 0. 009）,but positively correlated with N-cadherin expression（r =0. 361,P ﹤ 0. 001）. Conclusion YB-1 may promote the occurrence and development of CRC by participating in EMT program.