核酸与ELISA检测联合应用于血液筛查的结果分析

Analysis of nucleic acid test and routinely ELISA test result for blood screening

目的分析核酸与ELISA检测联合应用于血液筛查的结果。方法使用ELISA检测对34 580份标本进行检测,阴性标本全自动核酸检测系统进行核酸检测。结果 33 364份常规检测阴性的标本中,共检出核酸有反应性的标本63份,经鉴别试验,检出42份HBV-DNA阳性标本,未检出HCV-RNA阳性和HIV-RNA阳性标本,检出阳性率达0.13%。结论 ELISA检测存在一定程度的漏检,核酸检测及规范化管理显得十分重要。

Objective To analyzse the results of nucleic acid testing and routinely ELISA test for blood screening. Methods 34 580 specimens were firstly detected by ELISA. All of the dual ELISA negative specimens were sent for nucleic acid test which were performed on automatic NAT system. Results NAT reactive specimens were 63 out of 33 364 ELISA negative specimens. The identification tests which used Roche detection reagents were performed. 42 samples were determined HBV-DNA positive. Meanwhile,no positive of HCV-RNA and HIV-RNA were detected. Conclusion There is a certain de- gree of residual rick by using only ELISA on blood screening. As a result, it＇s very important for implement of nucleic acid test and operation of standardization management.