超短肠大鼠结肠代偿相关蛋白筛选与鉴定

Screening and identification of the colonic adaptation-related proteins in ultra-short bowel rats

目的 筛选并鉴定与结肠代偿相关的蛋白质.方法 SD大鼠20只,随机分为两组,每组10只.超短肠组：制作切除90％小肠的超短肠大鼠;对照组：横断小肠后再吻合.术后喂养21d,提取结肠黏膜蛋白质,通过双向电泳,建立各组蛋白质双向电泳图谱,对异蛋白斑点进行MALDI-TOF/TOF-MS鉴定,通过查找数据库分析差异蛋白功能.结果 建立了超短肠组、对照组结肠黏膜蛋白的双向电泳图谱,通过质谱技术鉴定出4个差异蛋白：前梯度同源蛋白2、丙酮酸激酶同工酶、蛋白酶激活剂复合物亚型1、胰甘油三酯酯酶,功能涉及糖和脂肪代谢、细胞增殖等.结论 鉴定出的蛋白质可能通过加强物质代谢、促进细胞增殖等途径参与结肠代偿.

Objective To screen and identify the colonic adaptation-related proteins.Methods Totally 20 SD rats were equally randomized into 2 groups：ultra-short bowel group （90% of intestine was resected） and control group （transection and then anastomosis of the intestine）.After 21 days of feeding,two-dimensional electrophoresis （2-DE） was performed to separate the total protein from the colon mucous epithelial tissues.Then Image Master 2D Platinum software was use to analyze differential expressional proteins,which were further analyzed by MALDI-TOF-MS and Mascot software to identify these proteins.Results The 2-DE patterns with high resolution were obtained from the colon mucous epithelial tissues of both groups.Four differentially expressed proteins were identified by MALDI-TOF-MS：M2-PK,AGR2,proteasome activator complex subunit 1,and pancreatic triacylglycerol lipase.These proteins were involved in sugar and fat metabolism and cell proliferation.Conclusion The identified proteins may play important roles in the process of colonic adaptation through substance metabolism and by stimulating cell proliferation.