长叶猕猴桃初始无菌组培体系建立

Establishment on Asepsis Tissue Culture System of Actinidia hemsleyana

[目的]建立长叶猕猴桃初始无菌培养体系。[方法]以长叶猕猴桃的带芽茎段、叶柄和叶片为外植体,以MS为基本培养基,展开三次双因子重复试验。[结果]长叶猕猴桃带芽茎段在MS+6-BA 1.00 mg/L+NAA 0.10 mg/L培养基中初始培养效果好;离体叶片小块在MS+6-BA 0.50 mg/L+NAA 0.10 mg/L培养基中初始培养效果好。[结论]为长叶猕猴桃组培苗的生产和工厂化快速繁育种苗奠定了基础。

[Objective] The aim was to establish an asepsis tissue culture system of A ctinidia hemsleyana. [Method] Taking the stem with shoots,leafstalks and leaves of A ctinidia hemsleyana Dunn. as explants and MS medium as the basic medium, the thrice double factors repeated experiment was carried out. [Result] The best initial medium for the stem with shoot of Actinidia hemsleyana was MS＋6-BA 1.00 mg/L＋NAA 0.10 mg/L; MS＋6-BA 0.50 mg/L＋NAA 0. 10 mg/L was the best initial medium for leaf segments of A ctinidia hemsleyana in vitro. [Conclusion] The results laided a foundation for somaclone production and factory rapid breeding seedlings of A ctinidia hemsleyana.