摘要
Adaptive optics is implemented in a confocal scanning fluorescence microscopy using a wavefront sensorless correction scheme. Using the image sharpness as the optimization metric, aberration correction is performed to compensate both system- and specimen-induced aberrations by using stochastic parallel gradient descent algorithm based upon Zernike polynomial modes. We demonstrate the idea of using phantom fluorescence samples experimentally. Enhanced imaging contrast and improved signal level are achieved.
Adaptive optics is implemented in a confocal scanning fluorescence microscopy using a wavefront sensorless correction scheme. Using the image sharpness as the optimization metric, aberration correction is performed to compensate both system- and specimen-induced aberrations by using stochastic parallel gradient descent algorithm based upon Zernike polynomial modes. We demonstrate the idea of using phantom fluorescence samples experimentally. Enhanced imaging contrast and improved signal level are achieved.
基金
This work was supported by tile National Major Scientific Equipment program （No. 2012YQ120080）. the National Science Foundation of China （No. 61108082）, the Sichuan Youth Science ＆ Technology Foundation （No. 2013JQ0028）, and the ／Vest Light Foundation of the Chinese Academy of Sciences.
