Th17/IL-17与呼吸道合胞病毒毛细支气管炎气道炎症相关性探讨

Correlation between Th17 cell,Interleukine-17 and airway inflammation after respiratory syncytial virus bronchiolitis

目的通过检测呼吸道合胞病毒(respiratory syncytial virus,RSV)毛细支气管炎(毛支)患儿外周血中Th17细胞及细胞因子IL-17的水平变化,探讨其与RSV毛支气道炎症的相关性。方法选择40例初次喘息发作的RSV毛支患儿作为研究对象,急性期及恢复期各抽取外周静脉血2mL,流式细胞术检测Th-17细胞比例,ELISA法检测IL-17水平。结果 RSV毛支急性期Th17及IL-17水平均明显升高(P<0.01);与恢复期相比,差异有统计学意义;重度毛支与轻度毛支相比,急性期Th17细胞比例及IL-17水平明显升高(P<0.01),而恢复期差异无统计学意义(P>0.05)。结论 Th17及IL-17可能参与RSV毛支急性期气道炎症反应过程,并与疾病的严重程度相关。

Objective To investigate the correlation between Th17 cell（Th17） ,Interleukine-17（IL-17） and airway inflammation through examining the concentration changes of Thl7 and IL-17 in serum of children who were suffering from respiratory syncytial virus （RSV） bronchiolitis. Methods Forty infants with the primary RSV bronchiolitis were recruited in this study. Two milliliter blood samples were collected in the acute phase and the recovery phase, respectively. The ratio of Thl7 cell was analyzed by flow cytometry,and the concentration of IL-17 was analyzed by and ELISA. Results The ratio of Th17 cell and the concentration of IL-17 in the acute phase of RSV bronchiolitis were both significantly higher than those in the recovery phase（P〈0.01）. Moreover, compared to children with mild RSV hronchiolitis, these two indexes in children with severe RSV bronchiolitis were both increased significantly in the acute phase （P〈0.01）, but no significant changes were detected in the recovery phase（P〈0.05）. Conclusions Th17 cells and IL-17 might be involved in the airway inflammation in the acute phase of RSV bronchiolitis,and be associated with the severity of the disease.