结肠癌微环境对骨髓间充质干细胞形态、增殖及CD_(13)、CD_(133)表达的影响

Influence of colon carcinoma microenvironment on cell morphology,proliferation and expressions of CD_(13) and CD_(133) of bone marrow mesenchymal stem cells

目的观察结肠癌微环境对骨髓间充质干细胞(BMSCs)形态、增殖及CD13、CD133表达的影响。方法对照组为BMSCs单独培养,实验组采用Transwell小室建立BMSCs与结肠癌SW480细胞非接触、共培养的微环境。显微镜观察BMSCs的形态变化,四氮唑兰比色法(MTT)检测BMSCs增殖情况,流式细胞仪检测其周期及表面抗原的表达。结果与对照组比较,实验组BMSCs的形态具备了恶性肿瘤细胞的特点,增殖速度增高,其S期细胞含量(41.1%)较对照组(9.67%)明显增加;实验组BMSCs的CD13的表达为89.7%±9.5%,明显高于对照组的67.1%±8.1%(P<0.01);实验组BMSCs的CD133的表达为90.5%±6.4%,明显高于对照组的4.3%±1.2%(P<0.01)。结论应用Transwell小室可实现结肠癌SW480细胞和BMSCs非接触共培养,并能诱导BMSCs细胞发生恶性转化,其机制与引起细胞形态、增殖能力及细胞表面标记物等生物学特性的改变有关。

Objective To investigate the effect of Colon carcinoma microenvironment on cell morphology, proliferation and expressions of CD13 and CDI33 of bone marrow mesenchymal stem cells. Methods Control group was bone marrow de- rived stroma cells cultured in DMEM medium, experimental group was （BMSCs） SW480 cells and bone marrow derived stro- ma cell（BMSCs） co - cultured in transwell chamber. The cell morphology of BMSCs was observed by microscope, and the generation of BMSCs were detected by MTF method. Cell cycle and expressions of CD13 and CDI33 were analyzed by flow cy- tometry （ FCM ）. Results Compared with control group, BMSCs in experimental group had the feature of malignant tumor cells, the generation speed significantly quicker and the percentage of S phase was significantly more than control group, The expressions of CD13 in experimental group { 89.7 % ±9. 5 % ） were significantly higher than control group （ 67.1%± 8. 1% ） （ P 〈 0. 01 ） , and the expressions of CDm in experimental group （ 90. 5% ± 6.4% ） were significantly higher than control group{4. 3% ±1.2% ） （P 〈 0. 01 ）. Conclusion These findings suggested that transwell chamber can help to achieve co - cultivation of SW480 cells and BMSCs and induce malignant transformation of BMSCs cell, the mechanism could be related to the changes of bionomics such as cell morphology, reproductive activity and cell surface marker.