摘要
目的建立一种三磷酸胞苷二钠注射液中精氨酸的含量测定方法。方法茚三酮分光光度法，检测波长567nm。结果精氨酸在2～12μg·mL。范围内呈良好的线性关系，R^2=0．9982，标准曲线回归方程为：A=0．0788C＋0．0329。平均回收率98．96％（n=9），RSD为1．24％。结论此法结果准确，简便快速，可用于三磷酸胞苷二钠注射液申精氨酸的测定。
Objective To develop a method for the determination of arginine in Cytidine Disdoium Triphosphate Injec- tion. Methods Ninhydrin spectrophotometry based on ninhydrin reaction was studied at 567nm. Results There was a good linear relationship in the range of 2 - 12 μg. mL for arginine（ R^2 = 0. 998 2 ）. The standard curve equation was A = 0. 078 8C ＋ 0. 032 9. The average recovery was 98.96% with RSD of 1.24% （ n = 9 ）. Conclusion The method was accu- rate,simple and rapid,and it can be used for the determination of arginine in Cytidinn Disdoium Triphaue Injection.
出处
《药学研究》
CAS
2014年第9期522-524,538,共4页
Journal of Pharmaceutical Research
关键词
三磷酸胞苷二钠注射液
精氨酸
茚三酮
Cytidine Disdoium Triphosphate Injection
Arginine
Ninhydrin
