共振散射比率法对SDBS与奎宁相互作用的研究

Study on the Interaction of Quinine and SDBS by a Resonance Light Scattering Ratiometry

基于表面活性剂SDBS与奎宁的结合反应发展了一种测定奎宁的双波长共振散射比率法。在BR缓冲介质中,SDBS与奎宁发生反应,并于280.0nm和375.0nm两处特征波长处导致共振光散射信号大大增强。通过分别测定I280.0、I375.0和I240.0/I245.0来检测奎宁。当SDBS的浓度为4.0×10^-5 mol/L时,以I280.0和I375.0为测定点的单波长共振光散射方法所得到的线性范围和检出限分别为0.16～30!mol/L、16.3nmol/L和0.15～30!mol/L、14.9nmol/L。而用I240.0/I245.0的散射强度之比来测定奎宁时,其线性范围和检出限分别为0.011～70!mol/L和1.12nmol/L。表明双波长共振散射比率法明显优于前者。

A dual-wavelength resonance lighting scattering（DW-RLS）ratiometry is developed to detect Quinine based on its bindings with surfactant SDBS.In Britton-Robinson buffer controlled medium,SDBS could interact with Quinine and display significantly enhanced RLS signals.By measuring the RLS signals characterized at 280.0nm（I280.0）、375.0nm（I375.0）and the RLS intensity ratio（I240.0/I245.0）,respectively,Over a wide dynamic range of content could detect Quinine.Typically,when SDBS concentration is kept at 4.0×10^-5 mol/L,using I280.0and I375.0could detect Quinine over the range of 0.16～30!mol/L、0.15～30!mol/L and with the limit of 16.3nmol/L、14.9nmol/L respectively,while using I240.0/I245.0could detect Quinine over the range of 0.011–70!mol/L with the limit of 1.12nmol/L.Showing DW-RLS ratiometry is obviously superior to the former one.