小鼠辅助性原位部分肝移植模型的建立

Auxiliary partial orthotopic liver transplantation in mice

目的探讨建立小鼠辅助性原位部分肝移植模型的技术方法。方法以C57BL／6小鼠为供、受鼠，制作小鼠辅助性原位部分肝移植模型50台／例，门静脉采用袖套法吻合，肝静脉采用端端丝线吻合，胆道采用支架胆肠吻合。总结手术技巧，并评估手术时间、手术成功率及长期存活率。结果供鼠手术时间、受鼠无肝期及受鼠手术时间分别为（30±3）min、（6±1）min和（58±5）min。手术成功率为96％，术后4周受鼠存活率为88％。结论小鼠辅助性原位部分肝移植模型的制备技术可靠，成功率高，可为辅助性原位部分肝移植的基础研究提供帮助。

Objective To explore the technique of auxiliary transplantation model in C57BL/6 mice. Method The donor portal vein partial orthotopic liver and hepatic vein were anastomosed with Cuff and suture techniques respectively. The donor bile duct was implanted into recipient duodenum. Result The operation time of harvesting donor＇s liver and anhepatic phase and recipient was （30 ± 3）, （6 ±1） and （58 ±5） min respectively. The model success rate was 96%, and the 4-week survival rate was 88 %. Conclusion The animal model was stable with high success-rate and can be used for the study of auxiliary partial orthotopic liver transplantation.