子宫颈鳞状细胞癌中Wnt-1和β-catenin的表达及其与转移的关系

Expression of Wnt-1 and β-catenin in cervical squamous cell carcinomas and relationship with metastasis

目的探讨Wnt-1、β-catenin蛋白在子宫颈鳞状细胞癌(cervical squamous cell carcinoma,CSCC)中的表达及其与侵袭转移的关系。方法采用免疫组化EliVision法检测78例CSCC组织和30例正常子宫颈组织中Wnt-1、β-catenin蛋白表达。结果在正常子宫颈组织中,Wnt-1、β-catenin蛋白的阳性率分别为20.0%和10.0%;CSCC组织中,Wnt-1、β-catenin蛋白的阳性率分别为56.4%和74.4%,二组差异有统计学意义(P<0.001)。33例CSCC淋巴结转移中Wnt-1、β-catenin蛋白的阳性率分别为72.7%和90.9%;45例CSCC无淋巴结转移中Wnt-1、β-catenin蛋白的阳性率分别为44.4%和62.2%。Wnt-1、β-catenin蛋白表达与CSCC的分化程度(P<0.05)、浸润深度及FIGO分期均有关(P<0.05),与患者年龄、肿瘤大小等均无关。Spearman相关性分析显示:Wnt-1与β-catenin蛋白表达呈正相关关系(rs=0.490,P<0.001)。结论 Wnt-1、β-catenin蛋白的表达异常可能参与CSCC的发生、发展及转移,Wnt-1、β-catenin蛋白均可作为预测CSCC侵袭、转移的潜能及临床预后的指标。

Purpose To investigate the expression of Wnt-1 and β-catenin protein in cervical squamous cell carcinomas（ CSCC） and their relationship with invasion and lymph node metastasis. Methods Expression of Wnt-1 and β-catenin protein were examined on immunohistochemistry containing 78 specimens of CSCC and 30 specimens of normal cervical tissues. Results The positive rates of Wnt-1 and β-catenin protein in normal cervical tissues were 20. 0% and 10. 0% respectively. The positive rates of Wnt-1 and β-catenin protein in CSCC were 56. 4%,and 74. 4% respectively. There was a significant difference between the two groups（ P〈0. 001）.The positive rates of Wnt-1 and β-catenin protein in 33 cases with lymph node metastasis of CSCC were 72. 7% and 90. 9% respectively. The positive rates of Wnt-1 and β-catenin protein in 45 cases with no lymph node metastasis of CSCC were 44. 4% and 62. 2% respectively. The expression of Wnt-1 and β-catenin protein was significantly related with grades of tumors and depth of invasion and FIGO stages（ P〈0. 05）,and there was no significantly difference between the expression of Wnt-1 and β-catenin protein and CSCC patients age（ P〈0. 05）. Spearman analysis showed that the expression of Wnt-1 protein was positive related to the expression of β-catenin protein（ rs= 0. 490,P〈0. 001）. Conclusion The abnormal expression of Wnt-1 and β-catenin may be involved in initiation,development,invasion,and metastasis of CSCC,and it is suggested that Wnt-1 and β-catenin be considered as potential markers for invasion,metastasis,and prognosis.