子宫颈鳞状细胞癌中IMP3表达与MVD的相关性分析

Expression and association of IMP3 and MVD in squamous carcinoma of the cervix

目的分析子宫颈鳞状细胞癌(squamous carcinoma of the cervix,SCC)中胰岛素样生长因子ⅡmRNA结合蛋白3(insulin-like growth factorⅡmRNA binding protein 3,IMP3)表达与微血管密度(microvessel density,MVD)的相关性。方法采用免疫组化SP法检测正常子宫颈(normal cervical epithelium,NCE)、低级别子宫颈上皮内瘤变(low-grade cervical intraepithelial neoplasia,CIN-L)、高级别子宫颈上皮内瘤变(high-grade cervical intraepithelial neoplasia,CIN-H)和SCC组织中IMP3表达及MVD值,并分析二者与SCC的关系。结果 (1)IMP3在NCE、CIN-L、CIN-H和SCC组织中的阳性率分别为0(0/15)、0(0/11)、37.5%(9/24)和86.0%(43/50),差异有统计学意义(χ2=53.345,P=0.000);与NCE组相比,CIN-H、SCC组中IMP3阳性率差异均有显著性(P<0.008 3);与SCC组相比,CIN-L、CIN-H组中IMP3阳性率差异均有显著性(P<0.008 3);(2)子宫颈组织中MVD值随子宫颈病变的进展而增高,差异有统计学意义(F=145.968,P<0.01),各组之间MVD值差异亦具有显著性(P<0.05);(3)SCC组织中IMP3阳性率及MVD值与肿瘤分化程度、肌层浸润深度及淋巴结转移有关(P<0.05),与患者年龄无关(P>0.05),SCC组织中IMP3表达与MVD值具有相关性(rs=0.323,P<0.05)。结论 IMP3在SCC的发生、浸润和转移过程中起重要作用,其异常表达可能与肿瘤的微血管形成有关。

Purpose To investigate the expression of insulin-like growth factorⅡ mRNA binding protein 3（ IMP3） and microvessel density（ MVD） in squamous carcinoma of the cervix（ SCC） and analysis their relationship in SCC. Methods The expression of IMP3 and MVD was examined by immunohistochemistry SP method in normal cervical epithelium（ NCE）,low-grade cervical intraepithelial neoplasia（ CIN-L）,high-grade cervical intraepithelial neoplasia（ CIN-H） and SCC. Results（ 1） The positive expression rates of IMP3 in NCE,CIN-L,CIN-H,SCC tissues were 0（ 0/15）,0（ 0/11）,37. 5%（9/24） and 86. 0%（43/50）,the difference was statistically significant（ χ2= 53. 345,P = 0. 000）. IMP3 expression was significant difference among NCE and CIN-H,SCC（ P 0. 008 3）,and that was also among SCC and CIN-L,CIN-H（ P〈 0. 008 3）.（ 2） The MVD count was increased with the development of cervical squamous lesion,there was significant difference among those groups（ F = 145. 968,P〈0. 01）,and the difference was also statistically significant between every two groups（ P〈0. 05）. The positive expression of IMP3 and MVD count in 50 cases of SCC tissues showed statistical difference in different pathologic grade,lymph node metastasis,and depth of tumor invasion groups（ P〈0. 05）,but didn＇t in different patients＇ age groups（ P〉0. 05）. And the positive expression of IMP3 was closely related with MVD count in SCC tissues（ rs= 0. 323,P〈0. 05）. Conclusion IMP3 plays an important role in the occurrence,infiltration and metastasis of SCC,and the abnormal expression of IMP3 may relate with the angiogenesis of tumors.