Toll样受体4在艾滋病相关型卡波齐肉瘤组织中的表达及其意义

The expression of Foil-like receptor 4 in the tissue of acquired immunodeficiency syndrome-related Kaposi sarcoma and its significance

目的：探讨 Toll 样受体4（TLR4）在艾滋病相关型卡波齐肉瘤（AIDS-KS）组织中的表达、分布情况和差异。方法收集2010年1月至2012年1月就诊于新疆医科大学第一附属医院的 AIDS-KS 患者11例、经典型卡波齐肉瘤（KS）患者7例，未并发 KS 的 HIV 感染者7例和健康志愿者7名作为对照，记录临床资料，收集病变区活组织检查标本，采用免疫组织化学法检测 TLR4表达。两组间均数比较采用 t 检验，等级资料采用U 检验，组间用单因素方差分析。结果TLR4在 AIDS-KS 和经典型KS 组织中呈强阳性表达，主要表达于异形血管内皮细胞和瘤细胞，定位在胞膜和胞质，同时发现在细胞核中也有表达；在未并发 KS 的 HIV 感染者和健康志愿者正常皮肤组织表达极少。TLR4在 AIDS-KS、经典型 KS、HIV 感染者和健康对照组的表达分别为0．242±0．443、0．208±0．059、0．072±0．042、0．065±0．031（F ＝33．70，P ＜0．01）。KS 患者 TLR4表达较非 KS 患者明显增强（t ＝4．097，P ＝0．003），但 TLR4在 AIDS-KS 与经典型 KS 组间差异无统计学意义（t ＝0．480，P ＝0．638），TLR4在AIDS-KS 中表达较 HIV 感染组高（t＝2．900，P ＝0．010）。结论TLR4在 AIDS-KS 和经典型 KS 组织中均高表达，推测 TLR4与 KS 发生、发展密切相关。

Objective To investigate the difference of expression and distribution of Toll-like receptor 4 （TLR4）between acquired immunodeficiency syndrome （AIDS）-related Kaposi sarcoma （AIDS-KS）and classic Kaposi sarcoma （KS）.Methods The subjects were collected from The First Teaching Hospital of Xinjiang Medical University from January 2010 to January 2012,including 11 patients with AIDS-KS,7 patients with classic KS,7 human immunodeficiency virus （HIV）infective patients without KS and 7 healthy volunteers.Clinical data and biopsy specimens were collected.The expression of TLR4 was detected by immunohistochemical method.Statistical analysis of mean between two groups was conducted by using t test.Statistical analysis of ranked data was conducted by using U test.Comparison between groups was analyzed with one-way ANOVA.Results The expressions of TLR4 were both strongly positive in patients with AIDS-KS and classic KS,and mainly in alien vascular endothelial cells （VEC）and tumor cells.TLR4 was located on cell membrane and cytoplasm,and also in the nucleus. TLR4 was rarely expressed in the skin tissue in HIV infective patients without KS and healthy volunteers. The expressions of TLR4 in AIDS-KS,classic KS,HIV infective patients and healthy volunteers were 0.242±0.443,0.208±0.059,0.072±0.042 and 0.065 ±0.031 ,respectively （F =33.70,P 〈0.01 ）. The expressions of TLR4 were lower in HIV infective （AIDS ） patients without KS and healthy volunteers.The expression level of TLR4 was significantly higher in KS patients compared with non-KS patients （t=4.097,P =0.003）.However,TLR4 expression had no significant difference between AIDS-KS and classic KS patients （t =0.480,P =0.638）.The expression of TLR4 was significantly higher in AIDS-KS patients compared with HIV infective patients （t=2.900,P =0.010）.Conclusions The study identifies that TLR4 is highly expressed in patients with AIDS-KS or classic KS.It is suggested that TLR4 may play an important role in occurrence and development of KS.