高效液相色谱法测定疲王按摩乳中阿魏酸的含量

The Content Determination of Ferulic Acid in Piwang Massage Lotion by High Performance Liquid Chromatography

目的建立疲王按摩乳中阿魏酸的含量测定方法。方法采用高效液相色谱法，色谱柱为Diamonsil C18（150mm×4．6mm，5μm，北京迪马科技有限公司）；流动相为甲醇．2％冰醋酸（32：68）；柱温为30℃；检测波长为320nm。结果阿魏酸在4．55～91．00μg／mL范围内呈良好的线性关系（R2=0．9998），其平均加样回收率分别为98．78％（相对标准偏差为2．668％）。结论该测定方法简便、快速、重现性好，可作为疲王按摩乳的质量控制方法。

Objective To establish a method for content determination of ferulic acid in Piwang massage lotion. Methods High performance liquid chromatography was used. The analysis was carried on Diamonsil C18 （150 mm× 4.6 mm, 5 μm, Dimma Science and Technology Co. Ltd.） column with mobile phase consisted of methanol-2% acetic acid （32 ： 68）. The column temperature was 30 ℃ . The detection wavelength was 320 nm. Results The responses of ferulic acid liner in range of 4.55-91.00 μg/mL （R2=0.999 9）. The average recovery of ferulic acid was 98.78 % （relative standard deviation is 2.668 %）. Conclusions The method is simple, rapid and good repeatability. It can be used for quality control of Piwang massage lotion.