IVF-ET后胚胎停育患者绒毛染色体异常的影响因素

Influencing Factors for Abnormal Molecular Karyotype of Villi Chromosome for Embryonic Arrest after IVF-ET

目的:探讨常规体外受精-胚胎移植(IVF-ET)后胚胎停育患者绒毛染色体异常的相关影响因素。方法:回顾性分析IVF-ET后胚胎停育的61个周期,按绒毛染色体单核苷酸多态性(SNP)分子核型检测结果分为分子核型正常组(A组)和分子核型异常组(B组),分析比较A、B组间对象的临床特征及实验室指标等。结果:未限制年龄时,女方BMI、流产次数、COH次数、不孕年限、女方基础FSH、Gn用量及获卵数A、B组间差异均无统计学意义(P>0.05),A组男方年龄、女方年龄、移植胚胎数小于B组,差异均有统计学意义(P<0.05),正常形态精子比率、头部缺陷精子比率、颈部和中段缺陷精子比率、尾部缺陷精子比率、胞质水滴缺陷精子比率、畸形精子症比例组间均无统计学差异(P>0.05)。女方年龄<35岁时,A、B组间上述各指标差异均无统计学意义(P>0.05)。结论:女方年龄与IVF-ET后绒毛染色体异常有关,精子形态学指标对IVF-ET后胚胎停育患者绒毛染色体分子核型检测结果无预测价值。

Objective： To study the influencing factors which result in abnormal molecular karyotype of villi chromosome for embryonic arrest after in vitro fertilization and embryo transfer （IVF-ET）. Methods： A total of 61 cycles of embryonic arrest after IVF-ET were retrospectively surveyed. They were divided into two groups based on the outcomes of single nucleotide polymorphism microarray （SNP array） in molecular karyotype analysis for villi chromosome： group A with normal molecular karyotype, group B with abnormal molecular karyotype. The paternal age, woman＇s body mass index （BMI）, infertility duration, the times of abortion, woman＇s basal FSH level, the number of controlled ovarian hyperstimulation, total gonadotropin dose, the number of oocytes retrieved, the number of embryo transfer and sperm morphology were compared between group A and group B. Results： Before controlling the woman＇s age, there was a significant difference bewteen the two groups in the woman＇s age, man＇s age and the number of embryos transferred （P〈0.05）, but there was no difference in the normal form rate, head defect rate, neck and midpiece defect rate, principal piece defect rate, excess residual cytoplasm （ERC） rate and teratozoospermia rate （P〉0.05）. After controlling the woman＇s age, there was no significant difference between the two groups in these indexes for the women less than 35 years old （P〉0.05）. Conclusion： There is a significant influence of woman＇s age on the outcomes of SNP array in molecular karyotype analysis of villi chromosome for embryonic arrest after IVF but not sperm morphology.