低频超声抑制人前列腺癌细胞PC-3侵袭的研究

Study of low-frequency ultrasound suppress invasion of human prostate cancer cell PC-3

目的 研究低频超声联合微泡抑制人雄激素非依赖型前列腺癌细胞侵袭及可能分子机制.方法 以频率21 kHz、声强为46 mW/cm2连续波超声辐照人前列腺癌PC-3细胞悬液30 s.分3组：对照组,单独超声组及超声联合微泡组（加声诺维200 μl）.辐照后,绘制细胞生长曲线;辐照后培养12 h,Transwell模型进行细胞体外侵袭实验,辐照后24 h实时定量PCR检测对基质金属蛋白酶-2、基质金属蛋白酶-9信使核糖核酸表达的影响.结果 辐照后各组细胞继续培养12 h,各组细胞生长区别不明显（均P＞0.05）;辐照后12 h,低频超声联合微泡组侵袭细胞数量明显少于对照组（P＜0.05）;低频超声联合微泡能够下调基质金属蛋白酶-2、基质金属蛋白酶-9的表达（P＜0.05）.结论 低频超声联合微泡能够抑制人雄激素非依赖型前列腺癌细胞PC-3的侵袭,并且可能通过下调基质金属蛋白酶-2、基质金属蛋白酶-9信使核糖核酸的表达来实现.

Objective To study whether ultrasound combined with microbubbles induces suppress invasion in androgen-independent prostate cancer cells PC-3 and to identify the probable mechanism.Methods Ultrasound with a frequency of 21 kHz and intensity of 46 mW/cm2 in continuous wave mode was used.Ultrasound combined with microbubbles （200 μl SonoVue） was used to treat androgen-independent human prostate cancer PC-3 cells for 30 seconds.PC-3 cells were divided into three groups：control group,ultrasound group and ultrasound combined with microbubbles group.Twelve hours after treatment,cell growth curve we drawn,and transwell chamber model was used to do cell invasion experiments in vitro.Real-time PCR was used to measure the expression matrix metalloproteinase-2 and matrix metalloproteinase-9 messenger ribonucleic acid.Results Twelve hours after treatment,cell growth was not significant difference among three groups （P 〉 0.05）.Twelve hours after treatment,ultrasound combined with microbubbles could significantly inhibit the invasion of human prostate cancer cells （P 〈0.05）.Treatment with ultrasound combined with microbubbles down-regulated the expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 messenger ribonucleic acid.Conclusions Ultrasound combined with microbubbles inhibited the invasion in human androgen-independent prostate cancer cells through down-regulation of matrix metalloproteinase-2 and matrix metalloproteinase-9.