豚鼠内淋巴囊经颅快速取材方法的研究

A Method for Fast Harvest of Guinea Pig Endolymphatic Sac

目的通过建立豚鼠内淋巴囊快速取材方法,为了解其对内耳功能的影响提供活性良好的组织材料。方法将健康成年豚鼠在显微镜下经颅内进行活组织解剖,并用摄像系统记录解剖过程及结果,组织学验证结果。结果在豚鼠颅骨标本上准确定位内淋巴囊位置,并在显微镜下成功解剖出豚鼠内淋巴囊,组织学证实解剖位置为内淋巴囊。结论经颅内解剖豚鼠内淋巴囊是简单易行、准确可靠的快速取材方法。

Objective To introduce a technique for fast harvest of guinea pig endolymphatic sac for study of inner ear functions changes. Method Transcranial dissection was conducted in alive healthy adult guinea pigs under a microscope and recorded on a camera system. Specimens were cross-examined histologically. Results The endolymphatic sac location was projected on the guinea pig skull, which successfully guided harvest of the endolymphatic sac under the microscope, with histo- logical confirmation. Conclusions Intraeranial vivisection of endolymphatic sac in guinea pig is a simple and easy technique.