枸橼酸盐透析液对慢性肾衰患者外周血Th17的影响

Effects of citrate dialysate on Th17 in peripheral blood of patients with chronic renal failure

目的探讨枸橼酸碳酸氢盐透析液对慢性肾衰(CRF)维持性透析患者外周血Th17的影响。方法选择维持性透析3个月以上且无活动性感染CRF患者57例,随机分为2组,分别给予枸橼酸碳酸氢盐透析液和普通碳酸盐透析液进行维持性透析治疗12个月,采用酶联免疫吸附法和流式细胞仪测定患者外周血白细胞介素-17(IL-17)含量水平及Th17亚群百分率。结果采用枸橼酸碳酸氢盐透析液的患者维持性血透6个月后外周血IL-17含量水平及Th17亚群百分率均明显低于普通碳酸盐透析液的患者;此外,枸橼酸碳酸氢盐透析液患者长期应用不良反应发生率明显低于普通碳酸盐透析液患者。结论枸橼酸碳酸氢盐透析液对CRF维持性血透患者的促炎症作用低于普通碳酸盐透析液患者,明显减少微炎症状态,临床值得推广。

Objective To investigate effects of maintenance hemodialysis with citrate-bicarbonate dialysate on Thl7 in peripheral blood of patients with chronic renal failure （CRF）. Methods The CRF patients （n = 57） with main- tenance hemodialysis for more than three months as well as free-active infection were selected and divided into twogroups randomly, which were treated by citrate-bicarbonate dialysate and ordinary carbonate dialysate for mainte- nance dialysis in 12 month, respectively. The levels of interleulin-17 （IL-17） and percentages of Th17 subgroup in peripheral blood were detected by enzyme-linked immunosorbent assay and flow cytometer, respectively. Results The levels of IL-17 and percentages of Thl7 subgro than six months were significantly lower than those up in patients treated with citrate-bicarbonate dialysate for more treated with ordinary carbonate dialysate. Moreover, incidences of adverse reactions in group of citrate bicarbonate dialysate were lower than those of common carbonate dialysate group. Conclusion The pro-inflammatory effects of maintenance hemodialysis with citrate-bicarbonate dialysate on the patients are lower than those of ordinary carbonate dialysate. Citrate-bicarbonate dialysate causes the reduction of inflammatory state, and is worthy of being popularized in clinic.