钛表面双层纳米管制备及生物活性的测定

The preparation of titanium surface coated with double-layers nanotubes and the determination of its biological activity

采用电化学两步阳极氧化法在钛表面制备纳米管,光滑纯钛作为对照组,通过场发射电镜、X射线能量色散谱和原子力显微镜观察分析试件表面微观形貌、元素组成和三维形貌并计算粗糙度。体外培养小鼠骨髓间质干细胞(BMSCs)进行生物活性的测定。结果显示在钛表面制备出有序的双层蜂窝状二氧化钛纳米管阵列,纳米管的表面由钛和氧元素组成,纳米管组粗糙度值大于光滑组,两组间比较差异有统计学意义(P<0.05),纳米管组试件促进了BMSCs的黏附、增殖和分化。

Two-step electrochemical anodic oxidation method was applied to prepare nanotube on titanium surface. Smooth titanium samples were regarded as a control group. Surface morphology, elemental composition, three-di- mensional topography, and the roughness were observed and analyzed by field emission scanning electron micro- scope, X-ray energy dispersive spectroscopy and atomic force microscopy, respectively. The mouse bone marrow mes- enchymal stem cells （BMSCs） were cultured to determine the biological activity in vitro. The results showed that double honeycomb titania nanotube arrays were successfully prepared. The elements in the surface of nanotubes composed of titanium and oxygen. The roughness of nanotube group was greater than that in smooth group with sig- nificant difference （P 〈 0. 05 ）. The surface of nanotube group promoted BMSCs adhesion, proliferation and differ- entiation.