摘要
为研究草酸在核盘菌致病过程中可能的作用,以模式植物拟南芥为材料,采用30mmol/L草酸喷施3周龄拟南芥,发现草酸显著诱导拟南芥AtWRKY63的表达。通过构建AtWRKY63过表达载体转化拟南芥,获得过表达AtWRKY63的纯系转基因植株,再用核盘菌活体接种拟南芥,结果表明过表达AtWRKY63植株对核盘菌的抗性显著增强。组织化学染色结果表明,AtWRKY63是通过诱导植物的氧爆发,抑制核盘菌菌丝的生长来抵御核盘菌的侵染;qRT-PCR对拟南芥转录水平分析表明,AtWRKY63可能激活了过表达植株的水杨酸与茉莉酸依赖的抗病信号途径,从而增强对核盘菌的抗性。
To explore the potential roles of oxalic acid （OA） in the pathogenesis of Sclerotina sclerotiorum,we sprayed 30 mmol/L OA evenly onto 3-week-old Arabidopsis and found AtWRKY63 was markedly induced by OA.To further elucidate the function of AtWRKY63,we obtained pure line of AtWRKY63-overexpression Arabidopsis by constructing AtWRKY63-overexpression vector and transforming Arabidopsis.The Arabidopsis plants overexpressing AtWRKY63 showed enhanced resistance to S.sclerotiorum.Histochemical staining revealed that overexpression of AtWRKY63 induced oxidative burst in host plants,which suppressed the hyphal growth of S.sclerotiorum,and consequently inhibited fungal infection.qRT-PCR analysis indicated that AtWRKY63 may involve in salicylic acid-dependent and jasmonic acid-dependent defense signaling pathway,thus,confers the resistance to S.sclerotiorum.
出处
《西北植物学报》
CAS
CSCD
北大核心
2014年第9期1721-1726,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(30671347)
福建省自然科学基金(2012J01079)
关键词
拟南芥
AtWRKY63
核盘菌
草酸
Arabidopsis thaliana
At WRKY63
Sclerotinia sclerotiorum
oxalic acid
