肝癌患者血清中miR-106、miR181含量检测及其与肝癌组织中Bax、Bcl-2的关系研究

Study on the serum content detection of miR-106, miR181 in hepatocellular carcinoma patients and its correlation with Bax, Bcl-2 level in tumor tissue

目的研究肝癌患者血清中miR-106、miR181含量及其与肝癌组织中Bax、Bcl-2的关系。方法将2011年1月~2014年1月在我院确诊并接受手术治疗的150例肝癌患者纳入研究的观察组,同期在我院体检的150例健康者纳入研究的对照组。采用real-time PCR的方法检测血清中miR-106、miR181含量,采用Western-blot的方法检测肝癌组织中Bax、Bcl-2的含量。结果观察组血清中miRNA-181b的相对含量低于对照组、miR-106的含量高于对照组;肝癌组织中Bcl-2的相对含量高于癌旁正常组织、Bax的含量低于癌旁正常组织;miR-106与Bcl-2含量呈正相关、与Bax含量呈负相关;miR-181与Bcl-2含量呈负相关、与Bax含量呈正相关。结论肝癌患者血清中miR-106、miR181含量显著异常,且与凋亡相关分子Bcl-2、Bax的含量相关,miR-106、miR181可能是调控肝癌细胞增殖和凋亡的潜在分子机制。

【Objective】To study the serum content of miR-106, miR181 in hepatocellular carcinoma patients and its correlation with Bax, Bcl-2level in tumor tissue.【Methods】150 cases of healthy persons received health examination in the same period were enrolled in control group. Then miR-106, miR181 contents in serum were detected by real-time PCR; Bax, Bcl-2 levels in tumor tissue were assayed by Western-blot. 【Results】Relative content of serum miRNA-181 b of observation group was lower than that of control group, relative content of serum miR-106 was higher than that of control group; relative content of Bcl-2 in tumor tissue of observation group was higher than that of control group, relative content of Bax in tumor tissue of observation group was lower than that of control group; miR-106 was positively correlated with Bcl-2 content and negatively correlated with Bax content; miR-181 was negatively correlated with Bcl-2 content and positively correlated with Bax content. 【Conclusions】miR-106 and miR181 content in serum of hepatocellular carcinoma patients are significantly abnormal and correlated with apoptosis related molecules Bax, Bcl-2; miR-106 and miR181 may be a potential molecular mechanism to regulate proliferation and apoptosis of hepatocellular carcinoma cells.