雌激素对实验性变态反应性脑脊髓炎大鼠发病影响的研究

Study on effect of estrogen on experimental allergic encephalomyelitis in rats

目的探讨雌激素对实验性变态反应性脑脊髓炎(EAE)的影响。方法将30只大鼠随机分为EAE组及大、小剂量雌激素干预组,每组10只,制作EAE模型,大、小剂量雌激素干预组分别给予皮下注射苯甲酸雌二醇1 mg/kg/d、250μg/kg/d,连续10 d,观察各组的发病情况并采用HE染色观察脑和脊髓组织病理变化。结果大、小剂量雌激素干预组的临床症状均较EAE组轻,表现为发病率减少、潜伏期延长、进展期缩短、高峰期神经功能损害较轻。病理切片提示雌激素干预组脑和脊髓炎症细胞浸润明显减少,其中以大剂量组更明显。结论雌激素对多发性硬化动物模型EAE具有保护作用,且与剂量相关。

Objective To investigate the effect of estrogen on experimental allergic encephalomyelitis （EAE） in rats. Methods Thir- ty male rats were randomly divided into three groups： EAE model group, high-dose estrogen treatment group, and low-dose estrogen treatment group, with 10 rats in each group. The high- and low-dose estrogen treatment groups were subcutaneously injected with estra- diol benzoate 1 mg/kg/d and 250 μg/kg/d, respectively, for ten days. Clinical symptoms of the rats were observed, and the pathologi- cal changes in cerebral and spinal cord tissues were observed by HE staining. Results The high-and low-dose estrogen treatment groups showed milder clinical symptoms as compared with the EAE model group, including lower incidence, longer delitescence, shor- ter progression period, milder neurological dysfunction at the peak stage of disease, and reduced infiltration of inflammatory ceils into cerebral and spinal cord tissues. The protective effect was more obvious in the high-dose estrogen treatment group. Conclusions Estro- gen has a protective effect on the multiple sclerosis model of EAE, and the effectiveness is positively correlated with dosage.