30个InDels在中国三个民族的遗传学调查及法医学应用

Forensic genetics study of 30 InDel markers for forensic identification in three Chinese populations

目的调查Qiagen InvestigatorDIPplex试剂盒30个InDels多态性位点在中国汉族、藏族、维吾尔族人群中的群体遗传学数据,评估其法医学应用价值。方法采集汉、藏、维吾尔族各90名无关个体静脉血,提取DNA。使用3130xL毛细管电泳对该270份样品进行分型,通过统计计算相关的群体遗传学参数。结果实验得到270份样品的分型及基因型频率,30个InDels未明显偏离Hardy-Weinberg平衡及连锁平衡,在汉族、藏族、维吾尔族三个人群中的随机匹配概率分别为1.42×10-11、7.19×10-12、4.74×10-13,累积非父排除率(CPE)均大于0.995 1。结论该组插入缺失位点在中国的汉族、藏族、维吾尔族人群中具有较高的多态性,能达到较高的个体识别能力,可以作为现有STR检验体系的补充。

Objective To study 30 InDel polymorphisms （ Qiagen Investigator DIPplex kit） of three populations in China （Han, Tibetan, Uyghur） and evaluate its forensic application. Methods DNA sam- ples were extracted from Han （ n = 90）, Tibetan （ n = 90）, Uyghur （ n = 90） populations in China, genotype data of 30 InDels were obtained by 3130xl Genetic Analyzer, and population genetic parameters were calcu- lated. Results None of 30 InDels deviated from Hardy-Weinberg equilibrium and linkage equilibrium. The random match probabilities were 1.42 x 10 -1l, 7.19 x 10 -l2, 4.74 x 10 -13 for Han, Tibetan, Uyghur, re- spectively. The cumulative probabilities of exclusion （CPE） were greater than 0. 995 1. Conclusion This panel of InDels is highly polymorphic in three Chinese populations and can be served as a supplementary to the current STR system for individual identification.