吗啡及其代谢物在藏毒致死者体内分布初探

Postmortem distribution of morphine and morphine-glucuronide in death induced by drugs hiding inside the body

目的采用固相萃取、液相色谱一串联质谱（LC-MS／MS）检验方法，考察吗啡和葡萄糖醛酸吗啡（M3G）在一例体内藏毒致急性死亡者体内分布情况。方法提取死者心血、尿、胃内容物、肝、肾、脑等15种检材，经Waters HLB小柱固相萃取后，C18色谱柱分离，采用电喷雾电离（ESI）、多反应监测模式（MRM）检测目标化合物。结果所建方法在0．0l～101μg／mL浓度范围内线性关系良好，提取回收率大于75％。结果显示总吗啡含量（游离态＋结合态）在胃内容物中最高，其次是尿、‘肾，在心血、胃组织、肺和腺体中居中，脑组织和心脏含量最低。结论本例检验结果验证了胃内容物、尿液和肾脏等是该类中毒案件的理想检材，其分布规律也可作为体内毒品分析实验依据。

Objective To determine the contents of morphine and morphine-glucuronide in blood, urine, gastric content, liver, kidney, brain et al. in acute morphine-related death induced by hiding drugs in human body by solid-phase extraction and LC-MS/MS. Methods Morphines were enriched by Waters HLB solid-phase extraction and detected by Multiple Reaction Monitoring （MRM） of LC-MS/MS. Results The method showed satisfactory linearity in the range of 0.01 ～ 10 μg/mL. The extraction recovery was over 75 %. The results indicated that gastric content had the most morphine, followed by urine and kidney, brain and heart had the least drugs. Conclusion The results proved that gastric content, urine and kidney were ideal samples in this kind of cases. Moreover, the distribution pattern in this case also provided important data for the studies of drug analysis.