摘要
Rice blast is one of the most devastating diseases in the world and outbreaks occur frequently.The differences in protein expression between blast resistant and susceptible near-isogenic lines(NILs)of japonica rice var.Yunyin infected with Magnaporthe oryzae were analyzed using proteomics,indicated that 67 different proteins were identified from 75 obtained proteins using the matrixassisted laser desorption/ionization time-of-flight mass spectrometry technique.Seven specific expression proteins,43 up-regulated proteins and 17 down-regulated proteins were identified among the 67 proteins.The bioinformatical analysis demonstrated that these 67 different proteins were involved in many biological physiological processes including five proteins related to photosynthesis,25 proteins related to metabolism,six proteins related to antioxidants,10 proteins related to protein synthesis and modification,five proteins related to signal transduction,four proteins related to adversity stress and 12 non-functional proteins.These identified proteins were directly or indirectly related to stress.Five proteins related to different physiological processes were selected.Their cDNA sequences were predicted and their expression patterns were analyzed using real-time PCR,demonstrated that the genes would response to M.oryzae and the response blindingly different between blast resistant and blast susceptible NILs.
Rice blast is one of the most devastating dis- eases in the world and outbreaks occur frequently. The differences in protein expression between blast resistant and susceptible near-isogenic lines （NILs） of japonica rice var. Yunyin infected with Magnaporthe oryzae were ana- lyzed using proteomics, indicated that 67 different proteins were identified from 75 obtained proteins using the matrix- assisted laser desorption/ionization time-of-flight mass spectrometry technique. Seven specific expression proteins, 43 up-regulated proteins and 17 down-regulated proteins were identified among the 67 proteins. The bioinformatical analysis demonstrated that these 67 different proteins were involved in many biological physiological processes including five proteins related to photosynthesis, 25 pro- teins related to metabolism, six proteins related to anti- oxidants, 10 proteins related to protein synthesis and modification, five proteins related to signal transduction,four proteins related to adversity stress and 12 non-func- tional proteins. These identified proteins were directly or indirectly related to stress. Five proteins related to different physiological processes were selected. Their cDNA sequences were predicted and their expression patterns were analyzed using real-time PCR, demonstrated that the genes would response to M. oryzae and the response blindingly different between blast resistant and blast sus- ceptible NILs.
基金
supported by the NationalBasic Research Program of China(2012CB723003)
the National Natural Sciences Foundation of China(30871509)
the Fujian Provincial Sciences Foundation(2007J0005,2009J06011)
